The effect of varying tonicity on the anaphylactic and other reactions of plain muscle / by H.H. Dale.

  • Dale, Henry Hallett, 1875-1968.
Date:
[1913]
Catalogue details

Licence: In copyright

Credit: The effect of varying tonicity on the anaphylactic and other reactions of plain muscle / by H.H. Dale. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    The exact similarity of the curves obtained when the two horns were tested successively in solutions of the same composition, showed that any marked difference in the responses obtained with the two horns might with confidence be attributed to the only deliberate variation of the conditions, viz., the composition of the fluid in which the organ was suspended. The Ringer’s solution used for the controls was made up accord- ing to one of two formulae. The first was that which I used in my previously published experiments on the anaphylactic reaction, being Locke’s formula, with the exception that the calcium content was reduced. It is as follows: NaCl, 0.9; KC1, 0.042; CaCl2, 0.012; NaHC03, 0.05; dextrose, 0.1; water (dis- tilled in glass), 100. This will be referred to as Ringer 1. The second was modified from a formula given by Tyrode (7), and has proved an extremely effective medium for preserving the excitability of the isolated guinea-pig’s uterus, without the spontaneous development of high tonus or large rhythm. It may be mentioned incidentally that it serves admirably for the method of standardising pituitary extracts which Laidlaw and I recently described (8). It is essentially Locke’s solution, with the addi- tion of a small proportion of magnesium chloride. The mag- nesium appears to possess a marked power of restraining the development of irregular automatic rhythm. The formula is: NaCl, 0.9; KC1, 0.042; CaCl2, 0.024; MgCl2, 0.0075; NaHC03, 0.05; dextrose, 0.1; water (glass distilled), 100. This is referred to as Ringer II. Neukirch (9) has used Tyrode’s solution with like effect for preserving the regular rhythm and excitability of isolated intes- tinal loops from the rabbit. The results obtained with these two Ringer’s solutions, with varying proportions of additional sodium chloride, have been of the same general type. MODIFICATION OF THE ANAPHYLACTIC RESPONSE Fiiedberger and Hartoch’s protective action was obtained by injecting as much as 1 cc. of saturated sodium chloride intra-
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