The influence of the medium on the toxicity of certain alkaloids towards protozoa / by T.A. Henry and H.C. Brown.

  • Henry, Thomas Anderson, 1873-1958
Date:
[1923.]
Catalogue details

Licence: Public Domain Mark

Credit: The influence of the medium on the toxicity of certain alkaloids towards protozoa / by T.A. Henry and H.C. Brown. Source: Wellcome Collection.

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    were taken up in a capillary pipette, ra[)i(lly mixed in a depression on a porcelain slal), and thon transferred to a capillary hard-glass tu])e, which was sealed at both ends, and it was not until this [)rocediire was ado[)ted that consistent results were obtained. In the following experiments the protozoon used was a s[)ecies of (xlaucoma, a ciliate somewliat similar to, but smaller than, Paraimecium. f The culture of this organism was filtered through co:irse muslin and washed twice in tap-water by centrifuging at slow si)eed. For this culture, and for his advice and help, we are indebted to Dr. (h iNI. Wen YON. The reagents used were dissolved in distilled water, and equal volumes of the cultures and reagents were used throughout. Experimental Results. SusceptihLlity to Alkali.— (xlaucoma + distilled water -f N/50 NaOH. „ + „ + N/lOO „ „ + „ + N/150 „ 1.) minuR^s. All dead. Alive. Alive. S^isceptihility to Acid.— Glaucoma + distilled water + N/50 HCl. „ + ,. + N/lOO „ .. + „ + N/150 „ All dead. All dead. Alive. It might be thought that distilled water alone would injure the organism, bnt if one volume of washed Glaucoma was mixed with two volumes of distilled water and sealed in a hard-glass capillary tube, the organism was found to be alive and active twenty-four hours later. The following table shows the effect of exposing washed Glaucoma to the action of emetine hydrochloride, conessine hydrochloride, and quinine dihydrochloride in presence of (1) distilled water. Cd) dilute acids, and