Studies on immunisation : second series with appendices dealing with anti-typhoid inoculation, chemo-therapy, and statistical and other operations of induction / by Sir Almroth E. Wright.

  • Wright, Almroth, 1861-1947.
Date:
1944
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Licence: In copyright

Credit: Studies on immunisation : second series with appendices dealing with anti-typhoid inoculation, chemo-therapy, and statistical and other operations of induction / by Sir Almroth E. Wright. Source: Wellcome Collection.

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    were administered in some eases by the mouth, in others subcutaneously, and in others, again, both by the mouth and subcutaneously. In a third series of nine pneumonic cases which were treated by Dr. John Parkinson at the London Hospital there were three cases in which the pupils became very widely dilated under the influence of the drug. The doses of aethyl- hydrocupreinhydrochlorate here ranged between 0-5 grm. or less to 1-5 grm. daily. It will be appreciated that it was, in view of the two former experiences, out of question to apply the treatment to any large number of patients unless the con- tingent advantage from the bactericidal action of the drug proved to be such as altogether to outweigh this element of risk. This, by the consent of all those who have worked with the drug, is not the case, and the very poor bactericidal results obtained on the repetition of the opto- cliin in the case of the first and third patients in Experiment I] b look like a forewarning of this. APPENDIX III THE EFFECT OF SANOCRYSIN ON B. TUBERCULOSIS By It. M. FRY, M.R.C.S.. L.R.C.P.2 (From the Laboratories of the Inoculation Department at St. Mary’s Hospital, London) Mdllgaard (1924), as a result of bis experiments, states that sanocrysin in a concentration of 1 in 100,000 not only completely inhibited the growth of B. tuberculosis in cultures, but also prevented it from growing subsequently when removed from the influence of the drug. The technique used by him was as follows : Equal portions of the film from a glycerine broth culture of the bacillus were trans- ferred to a series of tubes of glycerine broth to which varying concentrations of sanocrysin had been added. After six weeks’ incubation there was no evidence of growth, and the films were then removed, washed, and transferred to glycerine broth containing no sanocrysin. After a further four weeks’ incubation in this medium the films failed to show any growth. It seemed advisable to put the microbes under more favourable conditions for growth, apart from the presence of the sanocrysin, and advantage was taken of the fact, demonstrated by .Sir Almroth Wright 3 that human blood orp lasma forms an excellent medium for cultivation of the tubercle bacillus. This has the further advantage that, using the special technique described by him, growth may be recognised under the microscope after only a few days’ incubation. The technique used was as follows : Tubercle bacilli from a culture on a solid medium were ground up in a Hayden’s mortar to break up clumps. They were then suspended in normal salt solution, and centrifuged rapidly for a few minutes to spin down any remaining clumps, and the supernatant emulsion was pipetted off and diluted to a suitable opacity, which had been roughly determined by previous 1 Vide p. 222. 2 Reprinted from the British Journal of Experimental Pathology, Vol. VII, 11120.