Testing for epinephrin (adrenalin) in blood : comparison of plasma and serum / by G.N. Stewart.

George Neil Stewart

Date:: [1912?]

Catalogue details

Licence: Public Domain Mark

Credit: Testing for epinephrin (adrenalin) in blood : comparison of plasma and serum / by G.N. Stewart. Source: Wellcome Collection.

6/12 page 506

effects in text-figures I to 3 might be underestimated by a reader who looks casually at the tracings. The uterus tests showed no constant difference between the various specimens. Experiment II.—Blood was obtained by puncture of an arm vein from F. W., a man aged 44 years, with mitral insufficiency and a history of lues several years ago.” Just before the vein was tapped, 0.04 gm. of hirudin was dissolved in 4c.c. of Ringer’s solution, and 22 c.c. of blood were then run into the solution. The test-tube was agitated constantly as the blood ran in so as to insure thorough mixture of the blood and hirudin solution. The flow of blood was free. The first portion coming through the cannula was rejected. No clotting whatever occurred, even in several days. As soon as the hirudin specimen had been obtained, 70 c.c. of blood were at once collected from the same man in another vessel, and allowed to clot. 13 c.c: of Ringer’s solution were added to it. Both specimens were immediately conveyed to the laboratory and put in the ice chest over night. Abundance of hemoglobin-free serum and plasma separated. At 11 A. M. the following morning tests were begun with an intestine prepa- ration from a rabbit. The preparation weighed 0.44 gm. The smaller appa- ratus was used. Observation 9 (text-figure 4) shows the effect of replacing Ringer’s solution by serum from the clot, and 11 the effect of re- 7T am much indebted to Dr. Bucher, of the City Hospital, for aiding me in collecting this material. While Dr. Bucher attended to the tapping of the vein, reat, ‘ . e = ae I gave undivided attention to the proper collection of the hirudin blood.

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