Handbook on contagious and infectious diseases in animals / issued by the Quartermaster General's Branch, Army Headquarters, India.

Date:
1936
Catalogue details

Licence: Attribution 4.0 International (CC BY 4.0)

Credit: Handbook on contagious and infectious diseases in animals / issued by the Quartermaster General's Branch, Army Headquarters, India. Source: Wellcome Collection.

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    When taking swabs from pustules, etc., of dead animals, the overlying skin should be more effectively sterilised by cauterising it with a red hot spatula or other suitable instrument. Peritoneal Fluid. When the abdominal cavity is opened, separate the bowels and insert the swab between the folds into the fluid, taking care to avoid touching any part of the viscera which have been handled or are likely to have been contaminated. The succeeding steps in the process are the same as 4 and 5 above (Wound dis- charges). Tissue. Smears.—Cut out a small piece of the organ, or bisect the nodule or lesion, and holding it in a pair of forceps, rub the cut surface of the tissue on the middle third of the slide, leaving the ends blank. If only a few cells adhere to the slide, scrape the cut surface with a scalpel, and repeat the spreading process. Caseat- ing and similar material from lesions can be spread — in the manner described for pus. Do not stain or fix. Dry, label and wrap the slides in paper. Rabies.—See chapter on Rabies. Interna] Parasites. Collect, wash in water and immerse in warm 70 per cent. alcohol (Absolute alcohol or Methylated spirit 7 parts, water 3 parts). This kills the parasites, straight- ens them, and preserves them. External Parasites. Microscopic parasites may be collected direct, by means of the fingers or forceps. They should be placed in a small bottle and stoppered. Preservatives are not required.
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