Text-book of histology : including the microscopic technic / by Philipp Stöhr ; translated from the tenth German edition by Emma L. Bilstein ; edited, with additions by Alfred Schaper.

  • Philipp Stöhr
Date:
1904
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Licence: In copyright

Credit: Text-book of histology : including the microscopic technic / by Philipp Stöhr ; translated from the tenth German edition by Emma L. Bilstein ; edited, with additions by Alfred Schaper. Source: Wellcome Collection.

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    bichromate (io b) and 6 c.c. of the 2 per cent. osmic-acid solution (No. 22). It should be prepared shortly before it is to be used. 17. Cox-Golgi mixture.—This is prepared by pouring together 40 c.c. of a 5 per cent. solution of potassium bichromate, 40 c.c. of a 5 per cent. solution of corrosive Sublimate, 32 c.c. of a 5 per cent. solution of potassium chromate, and 88 c.c. of distilled water. This mixture may be kept in stock. 18. Ten per cent. phosphomolybdic acid.—Fifty c.c., kept in the dark. 19. Iron solution.—Dissolve 2.5 gm. of ferric alum—(NH4)0Fe,- (S04)4-in 100 c.c. of distilled water. 20. Picric acid.—Keep on hand 50 gm. of the crystals and 500 c.c. of a saturated aqueous solution, in which undissolved crystals in a stratum 2 to 3 mm. deep must always lie on the bottom of the bottle. It dis- solves readily. 21. Chromic-acetic acid.—To 50 c.c. of the 0.5 per cent. chromic- acid solution (9 b) add 50 c.c. of distilled water and from 3 to 5 drops of glacial acetic acid. 22. Osmic acid.—This may be obtained from the dealer—50 c.c. of a 2 per cent. solution—shortly before it is needed. It is very expensive. It should be kept in the dark or in a dark glass bottle and if well stoppered can be preserved many months. 23. Chromic-acetic-osmic acid (Flemming’s mixture).—Prepare a 1 per cent. chromic-acid solution (5 c.c. of the 10 per cent. solution [No. 9] to 45 c.c. of distilled water) and add 12 c.c. of 2 per cent. osmic acid and 3 c.c. of glacial acetic acid. This mixture is not injured by light and can be kept in stock.* 24. Platinuni chlorid.—Prepare a 10 per cent. stock solution, 2 gm. dissolved in 20 c.c. of distilled water. 25. Platinum-acetic-osmic acid mixture (Hermann’s mixture).—Pour into 60 c.c. of a 1 per cent. solution of platinum chlorid (6 c.c. of stock solution and 54 c.c. of distilled water) 8 c.c. of 2 per cent. osmic-acid solution and 4 c.c. of glacial acetic acid. 26. Saturated Sublimate salt solution.—Put 7.5 gm. ot common salt into one liter of distilled water; after solution add 125 gm. of crys- talline corrosive Sublimate and dissolve by the aid of heat. Filter the warm solution. On cooling, white acicular crystals form on the bottom of the bottle. 27. Silver nitrate.—A 1 per cent. solution (1 gm. of silver nitrate in *Tissues fixed in old Flemming’s fluid often stain badly, because the acetic acid bas evaporated; 5 to 20 drops of acetic acid newly added to the solution removes this defect.
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