The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.

  • Lee, Arthur Bolles, 1849-1927.
Date:
1885
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Licence: Public Domain Mark

Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.

Provider: This material has been provided by The University of Leeds Library. The original may be consulted at The University of Leeds Library.

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    CHAPTEE XLIV. ARTHROPODA. 612. Nerve and Muscle of Arctiseoida (Doyere's method^). —A score or so of Milnesium tardigradum are collected (it is well to have a large number, as the process hj no means succeeds with all individuals) and put into a test-tube with water that has been deprived of its air by boiling. A drop of oil is run on to the surface of the water, so as thoroughly to exclude the air. After twenty-four to forty-eight hours the animals will be found, not dead, but fixed and extended in a cataleptic state; the circulation of the perivisceral fluid has ceased, the pigment of the cuticle has disappeared or collected into patches that are no hindrance to observation, the entire animal has gained in transparency, and the ner- vous and muscular systems stand boldly out. The animals are examined in boiled water, unless it be wished to study the phenomena of resuscitation, in which case spring-water should be used. 613. Brain of Insects (Wagner's method^).—Harden in Betz's liquid (mixture of equal parts of sulphuric ether and chloroform), and make sections. 614. Striated Muscle (ThanJioffer's method^).—In order to demonstrate the two plates of the sarcolemma, digest muscle 1 'Arch. Mile. Aimt.,' i (1865), p. 105. ' Coinptes reiulus,' 1879 (2me serie), ]\ 379. 3 «Arch. Mile. Auat.,' -xxi (1882), p. 27.