Outlines of practical physiology : being a manual for the physiological laboratory, including chemical and experimental physiology, with reference to practical medicine / by William Stirling.

  • William Stirling
Date:
1902
Catalogue details

Licence: Public Domain Mark

Credit: Outlines of practical physiology : being a manual for the physiological laboratory, including chemical and experimental physiology, with reference to practical medicine / by William Stirling. Source: Wellcome Collection.

    40/690 (page 10)
    (b) It is partially soluble in acids and alkalies, when boiled. (c) Bruise some in water, and test with Millon’s reagent. (B) Proteids coagulated by ferment action. (i.) Fibrin is insoluble in water and weak solutions of NaCl. It is a white, fibrous, soft, and very elastic substance, which exhibits fibrillation under a high magnifying power (see “ Blood ”). (a) Place well-washed, chopped-up fibrin in a test-tube, add 0 2 p.c. HOI. It swells up and becomes transparent in the cold, but does not dissolve. On neutralising with dilute ]STa2C03 it regains its original size. (b) Repeat (a), but keep on a water-bath at 6o° C. for several hours ; filter, and test the filtrate for acid-albumin by neutralisa¬ tion with very dilute KHO or Na2C03. (c) To a very dilute solution of CuS04 add fibrin. The latter becomes greenish, while the fluid is decolourised. Add NaHO, the flock becomes violet. (d) To chopped-up fibrin suspended in water apply the xanthoproteic and Millon’s tests for proteids. (e) For the effect of a dilute acid with pepsin (see “Digestion”). These digest fibrin, and convert it into proteose, and ultimately into peptone. {f) It decomposes hydric peroxide, and turns freshly-prepared tincture of guaiacum blue (see “Blood”). (g) Digest fibrin in io p.c. NaCl for two days. A small part is dissolved ; boil the fluid — coagulation. (ii.) Myosin (see “Muscle”). (iii.) Casein (see “Milk”). (iv.) Gluten (see “Bread”). [VII. Combined or compound proteids consist of proteids com¬ bined with non-proteid bodies—e.g., haematin, carbohydrates, or nucleic acid. Haemoglobin (“Blood”). Histohaematin. Haemocyanin. Mucins. Mucoids. Oaseinogen (see “ Milk ”). Vitellm. N ucleo-histon.] [VIII. Protamines—regarded by Kossel as the simplest pro- (a) Chromo-proteids, (b) Gluco-proteids, . (c) Nucleo-proteids,