Outlines of practical physiology : being a manual for the physiological laboratory, including chemical and experimental physiology, with reference to practical medicine / by William Stirling.

  • William Stirling
Date:
1902
Catalogue details

Licence: Public Domain Mark

Credit: Outlines of practical physiology : being a manual for the physiological laboratory, including chemical and experimental physiology, with reference to practical medicine / by William Stirling. Source: Wellcome Collection.

    46/690 (page 16)
    from true mucins, which yield no nuclein. They react some¬ what like globulins and mucin. a Preparation.—As it is most abundant in nuclei it is best prepared from cellular organs—e.g., lymphatic glands, thymus. A. Sodium chloride method.—Grind finely-divided thymus gland in a mortar with an equal volume of NaCl and a little water. Pour the viscous mass into distilled water, when stringy masses rise to the surface. Collect and dissolve these in i p c. sodium carbonate solution. B. Grind up thymus or lymphatic glands of ox with sand, and extract with io to 20 vols. of water (i to 2 hours, better 24 hours). Strain, and, if possible, centrifugalise. Add acetic acid, dropwise, until acid, when the nucleo-proteid “ tissue fibrinogen” of Wooldridge is precipitated. (a) Dissolve the precipitate in 1 p.c. Na2C03, or dilute alkali = opalescent solution. (b) Boil (a) = no coagulation. The original solution in an acid medium is coagulated by heat. (c) Add acetic acid = precipitate of nucleo-proteid. (d) Saturate with MgS04 or Am2S04 = precipitate of nucleo-proteid. (e) It yields the ordinary proteid reactions. (/) Digested in gastric juice it yields an insoluble residue of nuclein, which when collected and boiled with HN03 and ammonium molybdate yields a yellow colour indicating phosphorus. (g) A few c. c. of the solution injected into the blood-vessels of a rabbit produce extensive intra-vascular clotting, especially in the veins. Record the results obtained with the following substances and tests :— Egg-Albumin Serum-Albumin. Serum-Globulin. Albumose. Peptone. Gelatin. Xanthoproteic, . Millon, .... CuS04 + NaHO, Nitric acid, Boiling and cooling, . C2H402 + K4FeCy6, . Tannic acid, HgCl2, .... Alcohol, .... Sat. with MgS04, ,, Am2S04, [Chemie der Eiweisskorper, by Otto Cohnheim, Braunschweig, 1900. The chemistry of proteid bodies, translated by Gustav Mann, M.D. Die Proteine, by G. Ruppel, Marburg, 1900.]