Tuberculosis of bones and joints / by N. Senn.

  • Nicholas Senn
Date:
1892
Catalogue details

Licence: Public Domain Mark

Credit: Tuberculosis of bones and joints / by N. Senn. Source: Wellcome Collection.

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    although not as intensive or brilliantly as with the more com- plicated solutions. In reference to the staining process, tubercle bacilli diftcr from all other known pathogenic microbes, in that they are penetrated very slowly by the aniline dyes, and in their specific behavior to decolorizing agents like mineral acids and alcohol. Upon what this differential behavior rests is as yet unknown. For section-staining Ehrlich's method is the best:— Saturated alcoholic solution of methyl violet or fuchsin, 11 parts. Aniline water, 100 Absolute alcohol, 10 Sections are left for twelve hours in this solution. Treat the specimen with 1:3 solution of nitric acid a few seconds. Wash in alcohol (60 per cent.) for a few minutes; after stain with diluted solution of vesuvin or methylene blue for a few minutes; wash again in 60-per-cent. alcohol, dehydrate in abso- lute alcohol, clear with cedar-oil, mount in Canada balsam. Ziehl-Neelsen's method has also been frequently employed ; 100 grammes of distilled water are mixed with 5 grammes of crys- tallized carbolic acid and 1 gramme of fuchsin, and to the filtered solution 10 grammes of alcohol are added. As a de- colorizing agent a 5-per-cent. solution of sulphuric acid is used. The remaining technique is the same as in Ehrlich's method. (Plate I, Fig. 2.) The examination of fluids for tubercle bacilli can be done rapidly and very satisfactorily by Gibbes' method. Gibbes' Magenta Solution. Magenta, . . .2 parts. Aniline oil, 3 Alcohol fspeciflc gravity 0.830), . . . . 20 Distilled water, 20 Stain cover-glass preparation in this solution for fifteen or twenty minutes; wash in (1 : 3) solution of nitric acid until the color is removed; rinse in distilled water. After stain with methylene blue, methyl green, iodine green, or a watery solution of crysoidin, five minutes; wash in distilled water until no more
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