The constituents of the flowers of Trifolium incarnatum / by Harold Rogerson.

  • Rogerson, Harold.
Date:
[1910?]
Catalogue details

Licence: In copyright

Credit: The constituents of the flowers of Trifolium incarnatum / by Harold Rogerson. Source: Wellcome Collection.

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    0-1360 gave 0’3752 C02 and 0-1588 H20. C = 75‘2; H = 12 9. C1GH3902 requires C = 75‘0; H = 125 per cent. „ C = 76-l; H = 12*7 The above fraction thus appeared to consist of a mixture of palmitic and stearic acids. Fraction 55—57°.—On recrystallising this fraction from glacial acetic acid, it yielded palmitic acid (m. p. 60—61°: Found, C = 74'8; H = 12’8. Calc., C = 750; 11 = 12*5 per cent.). Ethereal Extract of the Resin. Isolation of Trifolianol, C21H3402(0H)2. During the extraction of the resin with ether it was observed that a sparingly soluble substance had separated. This was collected, washed, and dried, wdien it amounted to 2*9 grams. As it contained some green colouring matter, it was first treated with alcohol, and subsequently recrystallised several times from dilute pyridine, when it was obtained in minute, colourless crystals, melt¬ ing and decomposing at 295—300°. It yielded colour reactions similar to those given by the phytosterols: 0-1014 gave 0*2662 C02 and 0*0948 H20. C = 7P6; H = 10-4. C2iH3604 requires C = 71*6; H = 102 per cent. A determination of its optical rotatory power gave the following result: 0'2944, made up to 20 c.c. with pyridine, gave aD — PIS7 in a 2-dcm. tube, whence [a]D — 44 1°. An acetyl derivative of the substance was prepared, and this, when crystallised from a mixture of ethyl acetate and alcohol, separated in flat needles, melting at 165—166°. It was analysed, and its optical rotatory power determined, with the following results: 0-1208 gave 0 3038 C02 and 0'0996 H20. C = 68‘6; H = 92. C21H3402(C0*CH3)2 requires C = 68*8; 11 = 9-2 per cent. 0*3122, made up to 20 c.c. with chloroform, gave a]} — 0°49/ in a 2-dcm. tube, whence [a]D — 26 1°. These results render it evident that the above-described substance is identical with trifolianol (compare Power and Salway, this vol., p. 249). The ethereal solution of the ethereal extract from which the trifolianol had been separated was evaporated, when 12 grams of a dark green resin were obtained. This was of an acidic nature, but yielded nothing of interest.
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