The microscopist : a manual of microscopy and compendium of the microscopic science : micro-minerology, micro-chemistry, biology, histology, and pathological histology / by J.H. Wythe.
- Joseph Henry Wythe
- Date:
- 1877
Licence: Public Domain Mark
Credit: The microscopist : a manual of microscopy and compendium of the microscopic science : micro-minerology, micro-chemistry, biology, histology, and pathological histology / by J.H. Wythe. Source: Wellcome Collection.
Provider: This material has been provided by the Francis A. Countway Library of Medicine, through the Medical Heritage Library. The original may be consulted at the Francis A. Countway Library of Medicine, Harvard Medical School.
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![Lime-water or baryta-water is used for investigating con- nective structures, especially tendon, as maceration en- ables the needle to divide its fibrilla. Chloride of Sodium.—Solutions of this salt for indifferent media should always have some colloid, as albumen or gum-arabic added (7.5 grains in 1000 grains of water for delicate structures). Bichromate of ])otash is used in stronger solution for the same purposes as chromic acid. MUllei'^s eye-fluid for hardening the retina, and preserv- ing delicate embryos, etc., consists of bichromate of potass,, 2 grammes ; sulphate of soda, 1 gramme ; distilled water, 100 grammes. Alcohol dissolves resins and many vegetable coloring matters ; renders most vegetable preparations more trans- parent, and albuminous animal tissues more opaque. Acetic acid and, cdcohol, 1 part of each to 2 of water, renders connective tissue transparent, and albuminoid tis- sue prominent. The proportions can be varied. Alcohol and soda (8-10 drops of strong solution of caustic soda to each ounce) renders many tissues very hard and transparent. Beale recommends it for embryonic struc- tures. Ether dissolves resins, oils, and fat. Turpentine renders dried animal sections transparent. Oil of cloves acts as turpentine. Solution of chloride of zinc, iodine, and iodide of potassium, is recommended by Schacht as a substitute for iodine and sulphuric acid to color vegetable cells, etc. Zinc is dis- solved in hydrochloric acid, and the solution is evaporated to syrupy consistence in contact with metallic zinc. This is saturated with iodide of potassium, iodine added, and the solution diluted with water. Wood cells, after boiling in caustic potash, are stained blue by it. JBoracic acid, used by Prof, Brucke to separate the ele- ments of red blood-corpuscles.](https://iiif.wellcomecollection.org/image/b21085225_0072.jp2/full/800%2C/0/default.jpg)
