Serum therapy; bacterial therapeutics and vaccines / [R. Tanner Hewlett].

  • Richard Tanner Hewlett
Date:
1903
Catalogue details

Licence: In copyright

Credit: Serum therapy; bacterial therapeutics and vaccines / [R. Tanner Hewlett]. Source: Wellcome Collection.

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    Preservation of the Anti-Serum After the blood has been collected, the flasks are allowed to stand in a cool place for two or three hours until clotting has occurred. It is a good plan then to give each flask a few sharp twists so as to detach the clot from the sides of the flask. The flasks are then allowed to stand in a dark, cool place for twenty-four to forty- eight hours until the serum has thoroughly separated, and the serum is then decanted off through the side tubule into sterilised bottles, in which it is kept until required. By some a small amount of an antiseptic is added—e.g. 0‘2 per cent, carbolic acid or, preferably. O'3 per cent, trikresol. Camphor, previously flamed to sterilise it, has also been used, but is only a feeble antiseptic. As a slight precipitate is apt to form in the anti-serum after it has been kept for a short time, rendering it turbid, it is usual to Alter the serum after it has stood, for a few days, and before bottling, through a Berkefeld filter, ar- ranged as for the filtration of toxin (p. 40). For distribution the anti-serum must be bottled aseptically. The bottles should be of about 10 c.c. capacity and be made of a non-
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