A precise investigation of some micro-organisms and soluble ferments, their chemical history and relation to disease : including also a practical study of the disinfecting value of 'Sanitas' fluids / by C.T. Kingzett.

  • Kingzett, Charles Thomas, 1852-1935.
Date:
1885
Catalogue details

Licence: Public Domain Mark

Credit: A precise investigation of some micro-organisms and soluble ferments, their chemical history and relation to disease : including also a practical study of the disinfecting value of 'Sanitas' fluids / by C.T. Kingzett. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    Saliva and the fermentation of Starch. Experiment I.—A solution of starch was made and then inoculated with a small quantity of saliva, after which the total solid contents were determined and found to amount to 1.732 per- cent, dry at 100° C. The following mixtures were then prepared and placed in open test glasses on July 13th :— A. 50cc. inoculated starch solution+50cc. water. B. 50cc. „ „ „ +25cc. „ +25cc. Sanitas. C. 50cc. „ „ „ -f-50cc. Sanitas. The Sanitas Fluid which was used in this experiment gave 5-902 per cent, total solid residue dry at 100° C, and contained matter which reduced Fehling's copper solution equal in amount to 2-7025 grm. grape sugar per lOOcc. On July 24th the solutions were made up to a known volume and chemically examined, with the results which are shown in the following synopsis :— Sugar originally Sugar present Difference due to Percentage of present. at end. action of ptyalin. starch converted. A. None 0-577 grm. 0-577 60 B. 0-6756 0-700 — — C. 1-44 1-3512 — — It is seen that in the absence of Sanitas the starch in the unprotected mixture was converted to the extent of 60 per cent, by the chemical change :— C6H10O5 + H,0 = CBH120„, whereas in the mixtures containing Sanitas practically no- change occurred, the slight differences in the quantity of sugar which were found present before and after, being within the limits of error of experiment. Experiment II. A further experiment was made with an inoculated starch solution, containing 4-472 per cent, matter dry at 100° C, and with another sample of Sanitas Fluid containing matter fixed at 100° C.=to 5-975 per cent., and reducing substance, which, calculated as grape sugar, amounted to 0-6493 per cent. -r the total acidity of the Sanitas being per lOOcc = to llocc. N/10 caustic soda solution.