Mammalian chimaeras / Anne McLaren.

  • Anne McLaren
Date:
[1976]
Catalogue details
    21/168 page 11
    Making eh imaeras 11 Table 1. The composition of some culture media used for embryo aggregation Mystkow- ska & Tarkowski, Mullen 1970 & (from Component Brinster, Whltten, Mulnard, Mulnard, Mintz, (g/1) 1965a 1971 1971 1967) 1964ö, 1971a used in different laboratories, is given by Biggers, Whitten & Whittingham (1971). Probably the most widely used technique is that introduced by Brinster (1963) in which embryos are grown in small drops of culture medium under mineral oil in a plastic Petri dish. The medium consists of a bicarbo¬ nate-buffered salt solution of the Krebs-Ringer type, supplemented with energy sources (pyruvate, lactate, glucose), bovine plasma albumin, penicillin and streptomycin, and the cultures are maintained in an atmosphere of 5 % carbon dioxide in air. Table 1 gives details of the medium and culture system used in some of the laboratories which have been most active in producing chimaeras. 2 M MC
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