On sugar in the urine / by H. Bence Jones.

  • Henry Bence Jones
Date:
[1862]
Catalogue details

Licence: Public Domain Mark

Credit: On sugar in the urine / by H. Bence Jones. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    acid given off by the yeast itself from tlie quantity given off by the yeast and sugar together? 2. What is the least quantity of sugar in water and urine that can be detected? 3. Wliat effect urea, oxalate of urea, and the residue of the urine have on the process of fermentation ? 4. What is the delicacy of the test as compared with the saccharimeter and Eehling's solution? 1. On the carbonic acid given off by yeast washed once with water:— 324*28 grns. of yeast gave 1*57 carbonic acid = 0*42 grns. percent. 421-50 „ „ ]-79 „ =0-48 In second experiment,— 240*70 grns. of yeast gave 0*75 carbonic acid = 0*31 per cent. 321*47 „ 1*00 „ = 0*31 „ 2. What is the least quantity of sugar in water and urine that can be determined? Half a grn. of grape-sugar with 29*99 grns, yeast, gave 0*39 grn. carbonic acid. 33*08 grns. yeast without any sugar being added gave 0*16 grn. carbonic acid. Therefore 29*99 grns. yeast gave 0*14 grn. carbonic acid. Hence, half a grn. of sugar gave 0*39—0*145 grn. carbonic acid = 0*24. Theoretically=0*24. In a second experiment, after the yeast had been well washed to remove every trace of alcohol, the following numbers were obtained:— Half a grn. of sugar, with 40*58 grns. of yeast, gave 0*34 carbonic acid. Without sugar, 52*27 grns. of yeast gave 0*06 .*. 40*58, give -046. Hence, half a grn. of sugar gave 0*34-0 05 =0*29 carbonic acid. The residue, after fermentation, was put into the smallest possible retort, and heated to boihng. The first drops that came over were tested for alcohol thus:—To one cubic centimetre of a moderately strong solution of bichromate of potassa, two or three drops of concentrated sulphuric acid were added, and then a few drops of the liquid supposed to contain alcohol. Heat was then gently used, and the fluid immediately became green from the alcohol present.