Text-book of histology : including the microscopic technic / by Dr. Philip Stöhr.

  • Philipp Stöhr
Date:
1903
Catalogue details

Licence: Public Domain Mark

Credit: Text-book of histology : including the microscopic technic / by Dr. Philip Stöhr. Source: Wellcome Collection.

Provider: This material has been provided by the Augustus C. Long Health Sciences Library at Columbia University and Columbia University Libraries/Information Services, through the Medical Heritage Library. The original may be consulted at the the Augustus C. Long Health Sciences Library at Columbia University and Columbia University.

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    cm. cubes) in a small glass jar containing freshly prepared Golgi's mix- ture (p. 21), which is to be covered and stood in the dark (in winter it must be put in an oven having a temperature of about 25° C). In from one to two hours the pieces can easily be cut into slices about 4 mm. in diameter. The quantity of Golgi's fluid to be used is regulated by the number of the slices, each slice requiring about 10 c.c. of the mixture. In from two to six days, less often fifteen days,* the slices are taken out, quickly washed for a couple of seconds in distilled water, gently dried with filter-paper, and placed in 0.75 per cent, silver solution (30 c.c. of the I per cent, solution [p. 22] plus 10 c.c. of distilled water, and for each piece 10 c.c. of this fluid).f A brown precipitate imme- diately envelops the pieces. They should be left in the silver solution for two days (which need not stand in the dark and must not be placed in the oven), and they may remain in it for six days without injury ; they are then placed for from fifteen to twenty minutes (not longer) in 20 c.c. of absolute alcohol, then embedded in elder-pith (or in celloidin, see Microtome Technic) and cut into thick sections. Each section should be at once examined, zvithont a cover-glass, with the low power, in order to ascertain its usefulness ; if it is good it is placed for from one to two minutes in a watch-glass containing abso- lute alcohol, then for a few minutes in carbol-xylol, then transferred to the slide. The xylol is removed by light pressure on the section with clean filter-paper and the preparation covered with a few drops of xylol- balsam. A cover-glass must not be applied, because it would prevent evaporation of the moisture in the section, which when retained destroys the Golgi preparations. Not infrequently, especially when the carbol- xylol has not been satisfactorily removed, the xylol-balsam gradually withdraws from the preparation, which in consequence appears Spoiled, but may be fully restored by the application of a fresh drop of balsam. At first the preparation should be examined with the low-power objec- tive ; when the balsam has become dry the high power may be used. until they are to be sectioned, and will keep in good condition for about ten months. Then transfer them directly into 95 per cent, alcohol for one hour; into alcohol-ether (equal parts) for a half hour ; into thin celloidin solution (in alcohol-ether) for one hour. Mount on a block with thick celloidin solution (see Microtome Technic) and harden in 80 per cent, alcohol for from one to two hours. Cut at once sections from 50 to loo /z thick ; clear them in a mixture of xylol, three parts, and carbolic acid, one part, in which they may remain for weeks without injury. Mount in balsam and cover the sections with a cover-glass. In time the specimens thus preserved are not infrequently marred by the appearance of corrosive crystals, but the impreg- nation of the elements of the nervous tissue remains intact. * See Special Technic. f The used Golgi mixture is to be thrown away.