The Erasmus Wilson lectures on the anatomy and pathology of the eye : delivered at the Royal College of Surgeons on Feb. 12th, 14th, and 16th, 1900 / by E. Treacher Collins.

  • Collins, E. Treacher (Edward Treacher), 1862-1937.
Date:
[1900]
Catalogue details

Licence: In copyright

Credit: The Erasmus Wilson lectures on the anatomy and pathology of the eye : delivered at the Royal College of Surgeons on Feb. 12th, 14th, and 16th, 1900 / by E. Treacher Collins. Source: Wellcome Collection.

Provider: This material has been provided by UCL Library Services. The original may be consulted at UCL (University College London)

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    by alternately sucking them into the pipette and expelling them into the tube (still surrounded by warm water in the incubator), and then i unit of the mixture is added rapidly (so that agglu- tination does not have time to take place again) to each tube of diluted serum. In making these additions, I am in the habit of making the first of each pair to the tube containing serum and extract, then to that containing serum and saline; by so doing I avoid carrying over a trace of complement to the second tube. In each case the corpuscles are very thoroughly mixed in. Agglutination Test. (Emery).—Macroscopic method. For a(iue<ius or blood serum. Materials Required.— i. Sedimentation tubes containing a known strength of an emulsion of the organism at the bottom of which is a bead of mercury.' 2. Aqueous or clear blood serum which has been collected in a pipette or blood capsule. 3. A fine ])iece (A capillary tubing having a known capacity in relation to the amount of emulsion in the sedimentation tubes. To Apply the Test.— Break off the top of the sedimentation tube containing the emulsion. Take the fine piece of capillary tubing with the known capacity and touch the surface of the blood serum or aqueous, which may have been blown out on to a clean slide so that the capillary tube becomes filled with the fluid. Drop the capillary tube containing the aqueous into the emulsion of the organisms and seal up the broken end of the sedimentation tube in the flame. When cool shake so as to make the bead of mercury mix the aqueous and emulsion of organisms together. Stand in a rack and beside it place a sedimentation tube containing an emul- sion of the same organisms. If the reaction be positive in the course of two to eight hours a more or less flocculent ]>recipitate will forni in the tube containing the aqueous, while in the other tube the organisms will settle down evenly to the bottom of the tube. 'For details, see Emery, Clinical Bacteriology and Hematology.