The relation of tetanus to the hypodermic or intramuscular injection of quinine / by Sir D. Semple. --.

  • Semple, David, Sir, 1856-1937.
Date:
1911
Catalogue details

Licence: In copyright

Credit: The relation of tetanus to the hypodermic or intramuscular injection of quinine / by Sir D. Semple. --. Source: Wellcome Collection.

Provider: This material has been provided by The University of Leeds Library. The original may be consulted at The University of Leeds Library.

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    CHAPIERI, A Series of Experiments retorileil in Tallies I to XIX. The 1st series of experiments.. In this series four guinea pigs were inoculated with washed tetanus spores under the skin of the hind leg, and next half a grain of quinine was injected hypoder- mically on the front of the chest, and repeated for three days. On the fourth day one animal developed tetanus, and died three days later. The other three animals remained well. Two control guinea pigs inoculated with '' washed tetanus spores only, remained well. Another control inoculated with 0'5 c.c. of the tetanus culture from which the spores were prepared developed tetanus on the fourth day, and died next day. It will be seen from this last experiment that the tetauns culture used was not very virulent, otherwise it would not have required four days for 0*5 c.c. to produce tetanus in a guinea pig. The results of tJie experiments recorded 'in this table show that when quinine is injected hypodermically into guinea pigs it produces conditions favourable for infection with washed tetanus spores, even when the spores are of low virulence. Table I. Kxperiiiieiits on (iuiiieji Fiji's to deuionstrati^ the effects of (Iniuiue iii producino' favour- able conditious for infection with Waslied Tetanns Spores. Avhen Spores are IHiven one day before <|uinjne. No. of ex- periment. Date of injec- ] Date of injec tion e 01 injec- \ JJate oi mjec- of spores. | tion of quinine. Amount of quinine injected. 4 5 and 6 (Control of spores.) 7 (Control of the culture from which spores were prepared.) 1st March 1910, right hind leg. Ditto Ditto Ditto Ditto 0-5 c.c. tetanus culture, 1st March. 2nd March 1910. 3rd March 1910. 4th March 1910. Ditto Ditto Ditto . on front of chest. Ditto Ditto Ditto Tetanus, 5th March. Died, 8th March. Remained well. Ditto. Ditto. Both controls re- mained well. Tetanus, 4th March. Died, 5th March. Symptoms local at outset, then became general. An anaerobic culture made from the tissues at the site of injection of spores grew tetanus bacilli. Symptoms local at first, then became general. The culture of tetanus used for this series of experiments was of low virulence, as evidenced by the fact that three days elapsed before |- c.c. produced tetanus in a guinea pig. (See No. 7 control experiment.) This series shows 25 per cent, of infections when spores were given one day before quinine ; the spores were injected in the hind leg, and quinine on front of the chest.