On the curative effect of carbonic acid gas or other forms of carbon in cholera, for different forms of fever, and other diseases / by C.J. Lewis.

  • Lewis, C. J. (Charles James), 1875-1937.
Date:
1903
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Licence: In copyright

Credit: On the curative effect of carbonic acid gas or other forms of carbon in cholera, for different forms of fever, and other diseases / by C.J. Lewis. Source: Wellcome Collection.

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    their cotton-wool plugs removed. The control plates and tubes incubated in a similar chamber in air were not uncovered and unplugged, because the air was not sterile and they would have hccomc contaminated. The carbonic acid gas, however, having been washed and passed through a sterile cotton-wool plug while freshly disengaged, was not likely to render cultures impure, and it was not found to do so. Experiment 1. To ascertain the effect of carbonic acid gas on cultures of Vibrio cholerte. Bacillus typhosus, and B. coli communis. The cultures were freshly inoculated from broth cultures which had been at 37° C. for 1 hour. A broth tube, a gelatine plate, and an agar plate of each organism were prepared, and set to grow in air containing 30 per cent, of carbonic acid gas. Exactly similar cultures were prepared and set to grow alongside the above, but in ordinary air. The temperature of incubation of both sets of cultures was 20° C. The growth is compared in the following table:— Time. Air. air and CO2. V.C. B.T. B.C.C. V.C. B.T. B.C.C. 2 <0 09 09 3 3 a 2 d 6 <40 d 09* 2 c3 3 •43 3 3 cd A cj Ph d A eS A CM CO PM d •4:3 0 0 0 Si Ak 0 U 09 c PM u 0 Si 09 C CM ti 0 ti 09 _C PM u 0 (h 09 A( n d W d bo < PP d tD < CP •4^ d CP eO bC < PP d ’S <! '3 *09 ’3 < 3 © < 0 0 0 0 0 C5 •5 00 00 03 2 09.2 00 09 •S 09 09 09 09 J fl S 3 *3 3 0 3 r: 3 a 3 d In 3 days. V P« & 0 0 S 0 a a Si 2 h 0 0, ■ J 0 ^ 0 s 0 a u 0 2 M a a Si 03 0 0 0 0 C 0 0 s.S 'A C 0 A A A 53 <73 c 3 'A 0 0 0 A A A o-^ s <0 'A A S 0 Jzi A !?; 0 ^ d ujA 0 V 09 >» s (d 09 3 *0 00 09 d C fc- 0 03 « Si ■S'? 0 *2 d IS 3 ’2 *2 3 3 In 7 days. a §3 0 » a ti 0 0 0 0 09 a a (h 0 a u 0 pH ••H 0 0 d •Sj <a C 0? e a ti 0 0 0 3 09 0 a u 0 0 5 s 'A 09 09 S21 A A 09 09 C9 .2-- & 09 09 A A 0 u 0 s V cc A4 d S ^0 0 Q p <4 <if Si 03 *3 *3 *3 ^ .s 0 § 09’.S 09 09 *2 0 3 3 3 3 3 3 In 14 days. C Si 0 >^4 *0 p-s Ad o> -C g 0 4^4 s u 0 a u 0 f^4 2 u 0 'A a u 0 >^4 2 0 A u 0 A 2 3 « 09 ^ 09 *0 C9 09 2 b 0 5^4 0 A a Ih 0 A a 0 A a ti 0 A a »-• 0 A 0 1“ ->! Jicsult.—Carbonic acid gas in this amount and at this temperature has little or no elfect on the B. typhosus or on the Bacillus coli communi.s. It has, however, a definite restraining influence on the Vibrio choleric, retarding its growtli in all media, and post- ],oiling both the liquefaction of gelatine and the formation of the usual ])cllicle on broth.