On the curative effect of carbonic acid gas or other forms of carbon in cholera, for different forms of fever, and other diseases / by C.J. Lewis.

  • Lewis, C. J. (Charles James), 1875-1937.
Date:
1903
Catalogue details

Licence: In copyright

Credit: On the curative effect of carbonic acid gas or other forms of carbon in cholera, for different forms of fever, and other diseases / by C.J. Lewis. Source: Wellcome Collection.

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    In 3 (fays.—TIic best gvowtli liatl taken place in the aerobic tnlie, and least in tlie anaei’oijic cnlture. In caiTonic acid tliere was now some turbidity tboiigli little ])cllicle. Result.—\.\xQ carbonic acid gas was insullicicnt to i-cstrain the organism altogether, though the first day’s growth was retarded. The restraining iidhience of CO, seems less potent at 37“ C. than at 20° or 24° C. 21 The effects of exclusion of oxygen were not the same as those of this amount of carbonic acid. Eoyperiment 4. To compare the growth on agar of Vibrio eholeras in air and carbonic acid at varying temperatures. Stroke agar ciiltures were used, one being set in air and the other in air containing 60 per cent, of carbonic acid. Both tubes were incubated at room temperature (16° C.). In 7 days.—The air tube showed normal streak growth, not so profuse as would have been the case at 37° C., but ample. The CO2 tube showed no growth. Both tubes were then removed to the incubator at 37° C., the one being incubated in air and the other in 60 per cent, of COg as before. They were thus kept for 7 days more. On the lAth day from, the commencement the air tube showed good streak growth. The tube maintained in carbonic acid showed no definite streak growth, but there was some growth now noticed in the broth fluid expressed from the agar, and lying near the foot of the agar tube. Rcstdt.—Carbonic acid gas in the proportion of 60 per cent, had practically restrained all growth of the comma bacillus, thougb it had not absolutely killed all the organisms. The higher temperature of incubation in the 2nd week assisted a few organisms to live. Probably at room temperature the tube would have remained sterile. Experiment 5. To compare the growth on agar and at blood-lieat of Vibrio cholera', B. typhosus, and B. coli communis in air and carbonic acid respectively. Agar stroke cultures were used and the tubes incubated at. 37° C. The percentage of carbonic acid gas was 60. In 24 honors.—The tubes in air were all growing well, but of those in the COg shows good growth. The others show oidy traces of growth. In 3 days.—\'\\Q cultures of V. cholenc and B. typhosus in air and COg sliow marked differences in extent of growth. In 9 da,ys.—The Vibrio cholcrcc has grown iis a narrow stri]> along the line of inoculation in the tube exposed to carbonic acid; its •rrowth is meagn; when coni])ared with that in air. The B. typhosios is not alike in the two tnbes. 'I hat in air shows a streak f)f small blue-grey rounded dots, isolated from (.•ach other towards the margins and end of the streak.