On the curative effect of carbonic acid gas or other forms of carbon in cholera, for different forms of fever, and other diseases / by C.J. Lewis.

  • Lewis, C. J. (Charles James), 1875-1937.
Date:
1903
Catalogue details

Licence: In copyright

Credit: On the curative effect of carbonic acid gas or other forms of carbon in cholera, for different forms of fever, and other diseases / by C.J. Lewis. Source: Wellcome Collection.

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    GUIJATIVE EFFECT OF CAEBONIC ACIJ) GAS The agar plates whicli had been in CO, and were apparently sterile N\eie now separated One was incubated in air, and tbe otber^n pure caibonic acid gas. The temperature of incubation was 26° C. The aear and broth tubes were continued in their respective jars. ° In 4 days (8 days from commencement):— Agar plates.—Neither tlie plate which had now been in CO, for 8 days, nor tbe plate wliich bad been in CO.^ for 4 days, and then in air for 4 days, showed any colonies of Vibrio choleric. Agar tubes and Broth tubes.—Much same as before. The carlionic acid gas was renewed and incubation continued. In 4 days (12 days from commencement):— Agar plates.—No colonies in either plate. Agar tubes.—Same as before. Broth tubes.—The broth tube in air is normal, that in COj shows no pellicle and no apparent growth, though perhaps not so clear as formerly. ' Incubation in carbonic acid gas was now discontinued, and all tbe plates and tubes incubated in air at 37° C. In 4 days (16 days from commencement):— Agar plates.—The plate wliich had been in COg 4 days and in air 12 days was sterile. The plate which had been in COg 12 days and in air 4 days showed four colonies which were cocci. These ^had probably gained access to the plate during a process of photography on the 12th day. Agar tubes.—The stroke culture which showed feeble growth during 12 days in carbonic acid developed well in air, and on subculture in broth and jelly proved to be Vibrio cholerie. In |he former subculture it gave the indol reaction with sulphuric acid alone. Broth tubes.—The tube which had been free from growth in carbonic acid for 12 days liegan to show signs of growth after 4 days in air, and after a fortnight in air at 37° C. was found to contain Vibrio cholerai, but even at that time it showed no pellicle or sediment which might have resulted from deposited pellicle. Result.—The arrest of cholera organisms was very marked, especially so in the agar plates and broth tube. The growth in agar stroke culture was exceptional, since in pure carbonic acid one would expect no growth, but certainly tbe gas seems to retard growth in agar plate more completely than in agar stroke culture. Subsequent incubation proved that a few organisms might survive in broth and be able to grow in it in a modified fashion under more favourable conditions. Experiment 11. To ascertain whether small ])ercentages of carl)onic acid gas in air l>roduced any noticeable ell'ects on the growth of Vil>rio cholerm and B. diphtheria;. CulLurtjy uwed: Agur iu pltilc uud tube, broUi, iiiid bluod-buruiii. I lie