The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy.

  • Arthur Bolles Lee
Date:
1890
Catalogue details

Licence: Public Domain Mark

Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy. Source: Wellcome Collection.

Provider: This material has been provided by The University of Leeds Library. The original may be consulted at The University of Leeds Library.

    401/440 (page 381)
    OTHKR METHODS FOR PROTOZOA. drop of water that contains tliem. But generally speaking it is best to employ only the vapour of the solution, exposing the organisms to its action for not more than from ten to thirty minutes). The objects having been covered, the excess of liquid is i-emoved by means of blotting paper, and the following stain is allowed to flow in : Glycerin 1 part. Water. . . . . . . 1 Picro-cai'mine . . . . . 1 „ (Eosin may also be used. Soluble anilin-blue does not give such good results.) The stain should be placed at the edge of the cover and the slide put away in a moist chamber in order that the water may evaporate very slowly and be changed very gradually for the glycei^iu-mixture ; if this pre- caution is not taken, shrinkage may occur. When the exchange has taken place, strong glycerin may be added and gi'adually substituted for the dilute glycerin. Certes states that the organisms thus prepared are fixed perfectly in their natural form, and allow of the study of the minutest detail of cilia, flagella, and the like, with the highest powers; the green colouration of EugleuEe and Paramecia is preserved. The nuclear structures are sharply brought out by the picro-carmine. 758. Saville Kent and Berthold [Manual of the Infusoria; Journ. Roy. Mic. Soc, 1883, p. 451) prefer a brownish-yellow solution of potassium iodide to osmic acid for fixing. 759. Demonstration of Cilia (Waddington, Journ. Roy. Mic. Soc, 1883, p. 185).— Solution of tannin, or a trace of alco- holic solution of sulphurous acid. 760. Other Methods for Protozoa. Maupas, Comptes rend., 1879, sem., p. 1276, and 2^ sem., p. 251.— (Fixation with alcohol followed by picro-carmine, glacial acetic acid, and glycerin). ])u Plessis, Vogt et Yung, Trait. Anat. Comp. Prat., p. 92.—(Fixation with02 per cent, solution of corrosive sublimate. Let the preparation dry up, and if the organisms have preserved their shape, stain, and mount in balsam). Geza Entz, Zool. Anz., 1881, p. 575.—(Fixation of the organisms in a watch-glass with liquid of Kleinenberg. Wash out with-alcohol, stain for