Immunoblotting.

The video explains the principles of immunoblotting with the aid of diagrams and demonstrates the technique in the laboratory. Immunoblotting explains why attempting to detect the presence of protein samples directly from an electrophoresis gel is impractical, due to diffusion of the protein bands over time and the fragility of the gel itself. The principles of immunoblotting are demonstrated and the transferring of information from the gel to a nitrocellulose membrane is described with the aid of diagrams. The techniques are then demonstrated in the laboratory by showing a method of making a single specific protein on the membrane visible using an antibody to that protein. The adding of a second antibody with an enzyme attached that will produce a coloured product when a substrate is added, is also unknown.