The use of mice in tests of immunity against yellow fever / by W.A. Sawyer and Wray Lloyd.

  • Wilbur Sawyer
Date:
1931
Catalogue details

Licence: Public Domain Mark

Credit: The use of mice in tests of immunity against yellow fever / by W.A. Sawyer and Wray Lloyd. Source: Wellcome Collection.

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    In the second supplementary test, the mice were inoculated intraperitoneally with the same amount of virus, French strain, 109th and 110th passages in mice, mixed with norma] human serum, but they were given also an intracerebral TABLE V Recovery of Yellow Fever Virus from the Circulating Blood of Mice at Varying Inter- vals after Massive Intraperitoneal Inoculation Hours between inoculation and bleeding of source animal 72 24 48 96 (1 day) | (2 days). | (3 days) | (4 days) | (5 oe (6 eee Principal Experiment, Series A No. of mice bled as source (blood 5 heparinized and pooled) No. of mice inoculated intracere- | 11 10 10 10 10 brally with the mixed blood and alive 3 days later No. of these mice dying or moribund 8 3 4 to 9 days after inoculation cn fa vos 2 — Nn 7 No. of mice bled as source (untreated 5 5 5 4 blood of each used separately) No. of mice inoculated in groups with | 25 22 these separate bloods and alive 3 days later No. of these mice dying or moribund 8 9 4 to 9 days after inoculation No. of mice bled as source (blood 6* pooled) . No. of mice inoculated with the | 11. mixed serum and alive. 3 days later oe _ No. of these mice agua or moribund 4 to 9 days affer inoculation o | 4 | | | | Principal Experiment, Series B | | ‘|. | Supplementary Experiments | | | | * First supplementary experiment. + Second supplementary experiment. injection of 0.03 cc. of 3 per cent starch solution to localize the virus and permit infection to take place. In this way it was hoped to find out whether the virus would disappear by the 6th day after injection, and, if it did, to ascertain whether it would reappear with the appearance of symptoms. Four of the mice which were
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