Mobilization and reassembly of genetic information : proceedings of the Miami Winter Symposium, January, 1980 / edited by Walter A. Scott [and others].

Date:
1980
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Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Mobilization and reassembly of genetic information : proceedings of the Miami Winter Symposium, January, 1980 / edited by Walter A. Scott [and others]. Source: Wellcome Collection.

    79/488 (page 55)
    A GENETIC ANALYSIS OF THE TRANSPOSON TN3 55 Type I Deletions: Simple insertion R388 ДТпЗ R388 D —T—□ IR ] IR BamHI Typen Deletions: Colntegrate formation R3e8 ДТпЗ pMB8 ДТпЗ R388 О H I V///Aï I I I I IR I IR IR I IR BamHI BamHI Fig. 3. Comparison of the sequences transposed Ъу com¬ plementation of type I and type II deletions. The deletions have transposed into the transmissable plasmid R388. Table IV. Dominance Test for Deletions of the Internal Resolution Site Plasmid(s) Freq. of Transposition Cointegrates ^Cointegrates were scored Ъу testing about 100 ampicillin resistant transconjugant colonies for colicin immunity. C. DM Sequence Analysis of the Mutants Affecting Tn3 Transposition The complete DNA sequence of Tn3 and 20 mutations which map within it has been determined. In the last section we described five mutations all of which mapped within a 500 bp region bisected by the BamHI site which showed an increase