Mobilization and reassembly of genetic information : proceedings of the Miami Winter Symposium, January, 1980 / edited by Walter A. Scott [and others].

Date:
1980
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Mobilization and reassembly of genetic information : proceedings of the Miami Winter Symposium, January, 1980 / edited by Walter A. Scott [and others]. Source: Wellcome Collection.

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    72 STANLEY N. COHEN et al. 35 40 45 50 |бО A-J-C-T-T-A-T-T-A-A-T-C-A-G-A-T-A-A-A-A-T-A-T-T-T-C-T-A-G-A- y.ft-Q-A-A-T-A-A-T-T-A-G-T-C-T-A-T-T-T-T-A-T-A-A-A-G-A-T-C-T- 190 Л-АЧ- jlT-T-C-A-'G-T-G-C-A-A-T-T-T-A-f-C-T-C-T-f-C-A-A-A^T-G-T-A-G- A-A-A-G-T-C-A-C-G-T-T-A-A-A-T-A-G-A-G-A-A-G-T-T-T-A-C-A-T-C- ТпЗ pTU4 ТпЗ Terminus --C-A-C-T-G-A ^ ^ G-T-C-A-6 ^ C-C-C-J 3 106 0 116 —1—— ] (;_д1 G-T-C-A-G C-C-C-CyA-T-A-C-G-A-T-,A-T С А 6-Л A-C-T ^ C-A-G-T-C G-G-G-G[T-A-T-G-C-T-A-T-A|T-T-C-A-A-C-A-T-T-ft-A 135 140 U5 150 15S lf>0 j_(;.ft-y-6-T-T-T-G-A-C-A-G-C-T-T-A-T-C-A-T-C-G-A-T-A-A-G-C-T- A-G-T-A-C-A-A-A-C-TìG-T-C-G-A-A-TtA-G-T-A-G-C-T-A-T-TTC-G-A- t t t 165 170 1'5 180 185 190 ,, T-T-A-A-T-G-C-G-G-T-A-6-T-T-T-A-T-C-A-C-A-G-T-T-A-A-A-T-T-G- A-A-T-T-A|C-G-C-C-A-T-C-A-A-A-T-A-G-T-G-T-C-A-A-T-T-T|A-A-C- 5' Figure 7. Partial DNA sequence of the transposase and repressor genes of Tn3 showing the inter—gene genetic control structures. These results provide a structural and functional basis for the previously reported regulation of transposition frequency of Tn3 (1), and also explain the observation that structural integrity of the region to the left of the BamHI site of Tn3 is required for transposition to occur (6-8). Since transposition is thought to be a multi-step process that involves DNA nicking and ligation steps, plus replication related functions, the large size of the Tnp peptide may reflect an ability to carry out multiple steps in the transposition process. PROMOTION OF REPLICON FUSIONS BY A TRANS-ACTING GENE PRODUCT OF THE F PLASMID Recently, it was reported that the tetracycline (Tc) resistance plasmid pSClOl can undergo integrative recombination (i.e., fusion) with a plasmid or bacteriophage genome that lacks extensive DNA sequence homology with pSClOl (1, 24-25), Such fusion of pSClOl with a second replicón can occur in the absence of the bacterial recA gene function, and appears to involve DNA sequences on pSClOl that resemble