Gene expression and development : the third of five volumes constituting the proceedings of the 4th International Congress on Isozymes, held in Austin, Texas, June 14-19, 1982 / editors, Mario C. Rattazzi, John G. Scandalios, Gregory S. Whitt.

International Congress on Isozymes

Date:: [cl983]

Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Credit: Gene expression and development : the third of five volumes constituting the proceedings of the 4th International Congress on Isozymes, held in Austin, Texas, June 14-19, 1982 / editors, Mario C. Rattazzi, John G. Scandalios, Gregory S. Whitt. Source: Wellcome Collection.

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$22 / Scandalios the amount of catalase protein, it was determined that this elevated catalase activity is concomitant with an increase in CAT-2 protein (Fig. 16). Turnover studies showed that these elevated CAT-2 protein levels are a consequence of increased levels of synthesis (Fig. 17). The developmental time course of catalase activity in reciprocal heterozygotes is intermediate to the parental patterns. Crosses were made to establish the inheritance pattern of this altered developmental program using a statistical analysis appropriate for this type W64A eoo E _з 5 400 э ь. S. 5 I 200 15 1 2 3 4 Б 6 7 8 9 10 11 12 13 Days after imbibition 12345678 DAYS AFTER IMBIBITION Fig. 15. The time course of catalase activity in W64A (( \) and R6-67 H >) scutella following germination. Scutella isolated from seedlings were ground with 25 mM glycylglycine buffer (pH 7.4). The homogenate was centrifuged at 17,000g for 30 minutes. The supernatant fraction was used for the catalase activity assay. (A) Catalase activity per mg of protein. (B) Catalase activity per scutellum. (From Scandalios et al [1980a], with permission.) Fig. 16. Rocket Immunoelectrophoresis with CAT-2 antiserum (35 fxl/gel) determining the amounts of CAT-2 protein in scutellar extracts of W64A and R6-67 during early sporophytic development. The height of each rocket illustrates the amount of CAT-2 protein present in each lane (one scutellum per lane). Note that the increased levels of catalase activity (Fig. 15) correspond to increased levels of catalase protein in line R6-67. Migration is anodal.$