Gene expression and development : the third of five volumes constituting the proceedings of the 4th International Congress on Isozymes, held in Austin, Texas, June 14-19, 1982 / editors, Mario C. Rattazzi, John G. Scandalios, Gregory S. Whitt.

International Congress on Isozymes

Date:: [cl983]

Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Credit: Gene expression and development : the third of five volumes constituting the proceedings of the 4th International Congress on Isozymes, held in Austin, Texas, June 14-19, 1982 / editors, Mario C. Rattazzi, John G. Scandalios, Gregory S. Whitt. Source: Wellcome Collection.

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$Gene Regulation During Development / 23 of study [Scandalios et al, 1980а]. Frequency distributions expected for the different homozygous genotypes and their heterozygotes were calculated for normal distributions from the means and standard deviations obtained for the parental lines and their F] progeny. Using these calculated values, points of minimum overlap among the distributions representing these three genotypes were determined as boundaries for classification. From these expected fre¬ quencies based on the normal distributions of the parental lines and the Fj crosses, expected frequencies were computed for the F2 and backcross gen¬ erations. The F2 and backcross data were then compared with the expected frequencies by goodness-of-fit tests. The results were consistent with the hypothesis that the elevated CAT-2 activity (and protein) observed in R6-67 Fig. 17. The rate of catalase synthesis (A) and degradation (B) in W64A (O) and R6-67 (•) scutella during the period after germination examined. Five scutella were incubated in 5 ml of 0.08 M 2-allyl-2-isopropylacetamide/0.2 M sodium acetate, pH 5.6, for 24 hr. The rate of catalase degradation was calculated by measuring the decrease of catalase activity per day per gram of scutellum (fresh weight) during the period of incubation. The amount of catalase synthesis was obtained from the sum of net synthesis and degradation during the incubation period. Kd, fraction of catalase units degraded per day; Ks, catalase units synthesized per scutellum per day. (From Scandalios et al [1980a], with permission.)$