Gene expression and development : the third of five volumes constituting the proceedings of the 4th International Congress on Isozymes, held in Austin, Texas, June 14-19, 1982 / editors, Mario C. Rattazzi, John G. Scandalios, Gregory S. Whitt.
- International Congress on Isozymes
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Credit: Gene expression and development : the third of five volumes constituting the proceedings of the 4th International Congress on Isozymes, held in Austin, Texas, June 14-19, 1982 / editors, Mario C. Rattazzi, John G. Scandalios, Gregory S. Whitt. Source: Wellcome Collection.
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![Gene Regulation During Development / 25 of chromosome 1. The scutella from ten-day-old seedlings were used in subsequent genetic analyses since the difference in ADH activity observed between the two inbreds is greatest at this point in development (Fig. 19). Detailed genetic and statistical analyses indicated that the altered develop¬ mental program of ADH-2 in R6-67 is due to a single gene with alleles exhibiting dominant-recessive inheritance [Lai and Scandalios, 1980]. At ten days, heterozygotes had an ADH activity level indistinguishable from that observed in W64A, suggesting that the rapid decrease in ADH-2 activity observed in W64A and in the Fi progeny is due to a trans-acting dominant allele at this locus. This regulatory gene exhibiting dominant-recessive in¬ heritance was designated Adrl (alcohol i/ehydrogenase-regulator) and is found to be unlinked to either Cat2 or Adh2 (Fig. 20) [Lai and Scandalios, 1980]. It should be noted here that, the identification of Carl and Adrl in R6-67 was merely fortuitous, because we have identified several other inbred lines 400| R6-67 X W64A 01 2345678 Days Post Germination Fig. 18. The developmental profile of scu- tellar ADH specific activity in W64A (#— —•), R6-67 (O O), and their Fj prog¬ eny (R6-67 X W64A) (Ö Ô). Pattern is same for reciprocal FjS. Points and bars rep¬ resent mean ± standard deviation in thrèe independent experiments. (From Lai and Scandalios [1980], with permission.) I ! W64 A Pi -r R6-67 ^2 oo§8§§888505880 (R6-67xW64A)xW64A BCi I I I r (R6-67xW64A)x R6-67 ВС? о §o8§8§§88§§8oooo (R6-67xW64A)® 00Í 50 s 5ooc о 8 0 50 100 150 200 250 30C 35C ADH ACTIVITY Fig. 19. Segregation of scutellar ADH activities in progeny from crosses between W64A and R6-67. Scutella were isolated from ten-day-old seedlings. Each circle represents one scutellum and was plotted along the horizontal axis according to its level of ADH activity (units per mg protein). (From Lai and Scandalios [1980], with permission.)](https://iiif.wellcomecollection.org/image/b18019742_0046.JP2/full/800%2C/0/default.jpg)
