Gene expression and development : the third of five volumes constituting the proceedings of the 4th International Congress on Isozymes, held in Austin, Texas, June 14-19, 1982 / editors, Mario C. Rattazzi, John G. Scandalios, Gregory S. Whitt.
- International Congress on Isozymes
- Date:
- [cl983]
Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Credit: Gene expression and development : the third of five volumes constituting the proceedings of the 4th International Congress on Isozymes, held in Austin, Texas, June 14-19, 1982 / editors, Mario C. Rattazzi, John G. Scandalios, Gregory S. Whitt. Source: Wellcome Collection.
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![a E — С ra ? 3 о Ф Q. 1 2 (J (О а> > _га О) Egg Larval Instar Pupa 1гладо Т он 1 ^ ISOZYME -GPOH 1 -GPDH 2 -GPDH 3 3 S С 7 S day» of development 4 ó 8 10 developmental program (days) 12 VA— 20 Fig. 1. a) The complete developmental program of GPDH expression in the inbred wild-type laboratory stock Samarkand. The time of expression of each isozyme (insert at top left) is illustrated by subjecting individuals at particular developmental times to starch gel electro¬ phoresis on a citric acid-histidine-NaOH gel at pH 7.0. b) The developmental expression of GPDH-specific CRM as determined by rocket immunoelectrophoresis. The samples represent 96-hour and 120-hour larvae (L), 168-hour and 216-hour pupae (P), and 12-, 24-, 48-, and 72-hour adults (A). From Bewley [1981], with permission. of GPDH activity in early pupae. During this entire developmental sequence, the only isozyme expressed is GPDH-3. In late pupae, approximately day 8, the enzyme activity declines to just detectable levels. This point in de¬ velopment marks the beginning of a rapid accumulation of enzyme activity that extends to day 12, when a steady-state level is reached in the adult that is three to four times the peak activity reached in late third instar larvae.](https://iiif.wellcomecollection.org/image/b18019742_0056.JP2/full/800%2C/0/default.jpg)
