Gene expression and development : the third of five volumes constituting the proceedings of the 4th International Congress on Isozymes, held in Austin, Texas, June 14-19, 1982 / editors, Mario C. Rattazzi, John G. Scandalios, Gregory S. Whitt.
- International Congress on Isozymes
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Credit: Gene expression and development : the third of five volumes constituting the proceedings of the 4th International Congress on Isozymes, held in Austin, Texas, June 14-19, 1982 / editors, Mario C. Rattazzi, John G. Scandalios, Gregory S. Whitt. Source: Wellcome Collection.
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![UF ► в| —GPDH1 p ^ ЩР i» -.-GPDH3 -( • ш WW adult larval lEF UF F S Fig. 3. Relationship between the electrophoretic mobility of naturally occurring alleles at the Gpdh^ structural locus and the major isozymes GPDH-1 and GPDH-3. a) Starch gel electro¬ phoresis of single adult flies (imagos) and larvae homozygous for the slow (S), fast (F), and ultrafast (UF) structural alleles illustrating coordinate mobility shifts for both GPDH-1 and GPDH-3. b) Enlarged portion of the fourth quadrant of a two-dimensional gel [O'Farrell, 1975] consisting of a crude adult extract of a line homozygous for GPDH'', doped with 5 |xg each of purified GPDH^-1 and GPDH^^''-!. Note the horizontal displacement of each variant in the isoelectric focusing dimension with relative pis of 6.3 (S), 6.1 (F), 5.9 (UF) and identical subunit molecular weights of Mr 32,000. that at least one subunit is common to all three isozymes, and it confers the electrophoretic properties just described. Crosses between flies bearing any two electrophoretic alleles give rise to heterozygous progeny with a complicated four or five banded pattern, de¬ pending on the resolution of the gel and the buffer system used, for GPDH activity on starch gels. All three isozymic forms possess bands of intermediate mobility in addition to the two parental forms in a dosage ratio of 1:2:1. In adult extracts, this dosage relationship is confirmed for GPDH-1 by pretreat-](https://iiif.wellcomecollection.org/image/b18019742_0060.JP2/full/800%2C/0/default.jpg)
