DNA repair mechanisms / edited by Philip C. Hanawalt, Errol C. Friedberg, C. Fred Fox.

Date:
1978
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: DNA repair mechanisms / edited by Philip C. Hanawalt, Errol C. Friedberg, C. Fred Fox. Source: Wellcome Collection.

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    DNA REPAIR MECHANISMS 9 A 549 BENZaA)PYRENE PLUS N-ACETOXY-ACETYLAMNOFLUORENE crû: IX Ii -я. Flg. 1 Sephadex LH-20 chromatography of enzymatic digests of DNA from A549 cells which were prelabeled with 32po-2 grown for 48 hrs. in the presence of 1.3^M [3H]benzo(a)pyrene and then treated with [l^C]N-acetoxy-acetylaminofluorene and incubated for 0 or 21 hrs. Ratios of the and radioac¬ tivity in each fraction over total radioactivity contained in the DNA digest are plotted in order to correct for DNA damage dilution due to cell growth. A]^, minor AAF lesion, AAF-(N2)dG; A2, major AAF lesion AAF-(Cg)dG; A^ (probably) deacetylated major AAF lesion AF-(C0)dG; B(a)P-diol- epoxide I-dG; В(a)P-diol-epoxide II-dG. BeN20(A)PYRENE PLUS N-ACETOXY- ACETYLAMINOFLÜORENE 10 20 Э0 40 50 HOURS POST-THEATMENT Fig. 2. Kinetics of excision from DNA of benzo(a)pyrene and N-acetoxy-acetylaminofluorene lesions in A549 cells. Data was derived from experiments of the type shown in Fig. 1 and the same symbols are used for the lesions. The horizontal lines mark the level of the individual lesions at the outset of post-treatment Incubation. Left side: B(a)P lesions (lesion Bq has not been chemically identified); right side: AAF lesions.
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