Cancer cell biology / edited by Takeo Nagayo, Wataru Mori.

Date:
[1980]
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Cancer cell biology / edited by Takeo Nagayo, Wataru Mori. Source: Wellcome Collection.

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    After cultivation (hr) Fig . 2. Detection of synthesis of cell surface-associated adhesive factor by AH136B cells using 125 I-labeled antibody against the factor. The synthesis begins to rise rapidly; it increases to 8-fold in 12 hr cultivation, reaching its peak, i.e., a 10-fold increase, in 24 hr cultivation. It is inhibited by actinomycin D or puromycin. O AH136B cells (lXl0 6 /50 ml in minimum essential medium (MEM)) ; ▲ AHI 36B cells (1 X 10 6 /50 ml in MEM containing 12.5 mg actinomycin D); A AH136B cells (lxl0 6 /50 ml in MEM containing 125 mg puromycin); • AH109A cells (1 Xl0 6 /50 ml in MEM). I) strongly suggested that this component may not be synthesized by the cells; only the serum-associated adhesive factor can be separated from the cells (22). As described above, although when the adhesive factor was exogenously applied AH109A and YS cells could form in vitro cell islands characterized by the development of junctional complexes, such adhering cells, like untreated AH109A and YS cells, proliferated as single cells when intraperitoneal^ inoculated {23). Furthermore, localization of the regenerated adhesive component was examined by the indirect immunofluorescence test using the immune IgG described above and FITC-labeled goat anti-rabbit IgG; preimmune rabbit IgG served as a control. Since the immunofluorescence test showed a specific binding of the antibody to the surface of the live recovered cells, the surface localization of this component is reasonable; the intensity and distribution of the immunofluorescence increased in parallel to the duration of cultivation (14, 21). The cells at 2 hr culture exhibited faint patch-like fluorescence at the limited small area of the cell surface (Photo 6A); those at 6 hr cul ture showed bright fluorescence at the larger (but limited) area of the cell surface