Reports of the trypanosomiasis expedition to the Congo, 1903-1904 of the Liverpool School of Tropical Medicine and Medical Parasitology / J. Everett Dutton, John L. Todd and Cuthbert Christy; with a comparison of the trypanosomes of Uganda and the Congo Free State / by H. Wolferstan Thomas and Stanley F. Linton; and supplementary notes on the tsetse-flies (genus 'Glossina', Wiedemann) / by Ernest E. Austen. Bound together with.

  • Dutton, Joseph Everett, 1877-1905.
Date:
1904-1905
Catalogue details

Licence: In copyright

Credit: Reports of the trypanosomiasis expedition to the Congo, 1903-1904 of the Liverpool School of Tropical Medicine and Medical Parasitology / J. Everett Dutton, John L. Todd and Cuthbert Christy; with a comparison of the trypanosomes of Uganda and the Congo Free State / by H. Wolferstan Thomas and Stanley F. Linton; and supplementary notes on the tsetse-flies (genus 'Glossina', Wiedemann) / by Ernest E. Austen. Bound together with. Source: Wellcome Collection.

Provider: This material has been provided by London School of Hygiene & Tropical Medicine Library & Archives Service. The original may be consulted at London School of Hygiene & Tropical Medicine Library & Archives Service.

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    III. TRYPANOSOMA DIMORPHON. GAMB1AN HORSE DISEASE This strain was brought back by Drs. Dutton and Todd from the Senegambia. Our results in general coincide with those of Dutton and Todd and Laveran and M ESNIL. Rats—White and Black The incubation varies ; in the case of the more attenuated strains the incubation period may be as long as twenty days ; with the ordinary strain, which has been passed through many rats, the incubation period is from three to twelve days, the average being from four to seven days. Duration of infection, seven to forty-two days ; average, eighteen days. Periodicity in some of the rats is fairly well marked. A white rat in the course of thirty-three days showed almost daily an irregular number of trypanosomes in the blood—after amounting to thirty to forty to a field they would diminish to one to thirty to forty fields, and then gradually rise. Each time when the parasites commenced to disappear a marked leucocytosis was present. On account of the smallness of the animal no blood count could be made. One rat showed a marked resistance to the parasite:—Experiment 285, rat (white), one hundred and eighty-five grammes weight. Inoculated intra- peritoneally January 5, 1904, with 0*75 c.c. of blood containing three trypano- somes to a field. Up to February 5, this animal never showed parasites in its blood. It was then reinoculated intraperitoneally with the whole blood from a rat (Experiment 242), there being two parasites to a field. Five days later tour parasites were seen to the cover-slip preparation. These continued present for three days ; its blood then became negative. On February 2 2, nine days later, it was once more inoculated intraperitoneally with 3*5 c.c. of pure blood from a dog (Experiment ] 1 4), there being twelve trypanosomes to a field in the mixture. It never became infected, nor was its blood infective to small rats when inoculated in quantities of o-5 c.c. to 0-75 c.c. If some of its serum was added to blood containing the parasites a marked and permanent agglutination occurred. Up to May 10 it was repeatedly inoculated with large quantities of virulent blood, it then succumbed to an epidemic of broncho- pneumonia. Unfortunately, very little serum could be obtained for agglutination and other work. Artificially a certain degree of resistance to this parasite can often be established by injecting a mixture of blood and citrate solution attenuated In- preserving in the incubator at 350 C. for tour to six hours. Out of seven white rats so treated five of them were able to withstand at the end of eighteen days inocu- lations of i*o to yo c.c. of virulent blood. Their blood, however, caused very slight d