Observations on some of the colouring matters of the bile and urine, with especial reference to their origin : and on an easy method of procuring haematin from blood / by C.A. Mac Munn.

MacMunn, Charles Alexander, 1852-1911.

Date:: [1883]

Credit: Observations on some of the colouring matters of the bile and urine, with especial reference to their origin : and on an easy method of procuring haematin from blood / by C.A. Mac Munn. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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$this place which was obtained by the action of trypsin on fibrin and treatment with oxidising reagents\ Chloroform solution. The chloroform solution was orange in colour and gave also a chlorophyll band in red and one between green and blue, but no band before this latter as in the case of the ether solution. On treatment with an acid the band between green and blue became quite black, on suitable dilution it was found to extend from A, 507 to 4865, but in a deeper layer it had a great breadth : in one experiment from X 507 to 462. The absence of unchanged bilirubin was proved by appropriate tests. Aqueous solution. The solution obtained by means of water was yellow in colour and gave only a double band at F, reminding one of the ether solution. The feeble part of this double band begins at X 519 and the dark part extends from X 503 to 484*5 with one drop of acetic acid. On treatment with zinc chloride a faint green fluorescence was seen and the zinc chloride band appeared from X 521*5 to 503. Rectified spirit and sulphuric acid. The solution obtained by means of rectified spirit acidulated with sulphuric acid was a dirty brown colour, and in a deep layer allowed only the red of the spectrum to pass; but in a thinner layer a band became detached at D, while in a still thinner layer a dark broad band became detached at the blue end of green. The first one read from X 613 to 582 and is that of indigo blue, while the dark band extended from about X 507 to 462, its darkest part being included between X 501 to 475. On diluting this solution with water and shaking in a separating funnel with chloroform and separating this off and filtering, a red soki- tion was obtained which gave the same two bands, the first from X613 to 585 and the second from X 509 to 4825. On pouring on a white dish the fluid at its edge had a dirty violet colour due to the presence of indigo-blue. On evaporating the chloroform solution on the water-bath a dark- brown, amorphous residue was obtained, which gave practically almost the characters of febrile urobilin both as to solubility and spectra of its solutions; these it is unnecessary to repeat. In none of the solutions could I detect unchanged bile-pigments. The position of the bands in an alcoholic solution with zinc chloride and with caustic soda, both of that close to C and at D, differs very slightly from that of those 1 Verhandlungen der Physikalisch-med. Gcsell. zu Wilrzhurg. N. F. B. xviii., Nr. 9. 1884.$