Essentials of bacteriology : being a concise and systematic introduction to the study of micro-organisms for the use of students and practitioners / by M.V. Ball.

  • Ball, M. V. (Michael Valentine), 1868-
Date:
1904
Catalogue details

Licence: In copyright

Credit: Essentials of bacteriology : being a concise and systematic introduction to the study of micro-organisms for the use of students and practitioners / by M.V. Ball. Source: Wellcome Collection.

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    the material and the growth occurs, or the medium is punctured with a stab of the platinum rod containing the material. The first is called a stroke or smear culture^ the second a stab or thrust culture. In removing the cotton plugs from the sterile tubes to carry out the inoculation, the plugs should remain between the fingers in such a way that the part which comes in contact with the mouth of the tube will not touch anything. After the needle has been withdrawn the plugs are re-inserted and the tubes labelled with the kind and date of culture. Plate Cultures. Several tubes of the culture medium are made liquid by heating in water bath, and then inoculated with the material as follows . A looped platinum needle is dipped into the material and then shaken in the tube of liquid media, (gelatine, agar, etc.). This first tube is called original. From this three drops (taken with the looped platinum rod) are placed in a second tube, the rod being shaken somewhat in the gelatine or agar; this is labeled frst dilution (a colored pencil is useful foiisuch markings). From the first dilution three dro])s are taken into a third tube, which becomes the second dilution. (Fig. 29.) The plugs of cotton must be replaced after each inoculation, and during the same must be carefully protected from contami- nation. To hasten the procedure and lessen the danger of contamina- tion, the tubes can be held in one hand aside of each other, each Fig. 30. Manner of holding plugs. plug opposite its tube. They are now ready for spreading on glass plates.