Lobsters, lymph and histamine-liberation / by W.D.M. Paton.

  • William Paton
Date:
1959
Catalogue details

Licence: In copyright

Credit: Lobsters, lymph and histamine-liberation / by W.D.M. Paton. Source: Wellcome Collection.

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    mine solutions containing adrenaline or noradrenaline in the dilution expected if the injected quantities were collected in the effluent. No increase (< 0.02 /^g./ml.) in histamine content of the effluent could be detected; and if adrenaline or noradrenaline, or the tem¬ porary vasoconstriction they produced, did release any histamine it cannot have exceeded 0.2 //g. Such a result implies that adrena¬ line and noradrenaline are of the order of 10,000 times less active than Compound 48/80, in releasing histamine, and may be totally inactive. 1 5i E 10- o> ZL *0 l_ c o o <u _c 105- X 0 I I I I-1 I » 1-1 ■ ' —I-1 I ■ ' I 0 20 40 60 80 100 120 140 160 180 200 220 240 t Volume Perfused (ml) Fig. 10. Cat’s isolated perfused skin. Output of histamine in response to 1 mg. tyramine. Img Tyramine Totol 59-8 mg A final set of experiments were made with certain other sym¬ pathomimetic amines, since the structure of Compound 48/80 can be regarded as that of a substituted polymerised tyramine. It was found that tyramine (fig. 10), ^-phenylethylamine and ampheta¬ mine could release histamine from the cat’s skin: 1 mg. tyramine mobilized 59.8 /ug. histamine; 10 mg. |^-phenylethylamine mobilized 121.7 jbLg. histamine; 10 mg. amphetamine, 12 jug. histamine. Methedrine (10 mg.) and propadrine (10 mg.), however, were nearly inactive (less than 1 jug. release), and ephedrine (10 mg.) appeared completely so. Intradermally, tyramine 0.1% and am¬ phetamine 1 % both produced wheals about 8 mm. in diameter and surrounding flares about 30 mm. diameter, but 0.1% ephedrine was indistinguishable from saline.
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