Comprehensive immunogenetics / W.H. Hildemann, E.A. Clark, R.L. Raison.

  • Hildemann, W. H., 1927-
Date:
[1981]
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Comprehensive immunogenetics / W.H. Hildemann, E.A. Clark, R.L. Raison. Source: Wellcome Collection.

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    Serotype Systems in Free-Living and Parasitic Protozoa 75 Fi Progeny t I /\ Syngen 1 90 G 60 G I ^ 5 fissions 60 G + 90 G Phenotype no interaction antigens 75 fissions 8 8 8 /\ /\ 8 8 8 8 r'N /N /N р2 Progeny II881BI8 Autogamy 5 fissions Segregation of pure 90 G + 60 G piienotypes in 1:1 ratio 8 8 8 8 -'N /N /N I I I I I I I 81ЙЙ111]! FIGURE 3-8. Segregation of allelic antigenic types 90G and 60G in Fl and F2 progeny. Source: Modified from Beale, 1954; also see Beale, 1974. temperatures. The G and D antigens were found to be clone-specific, as ev¬ idenced by immobilization reactions in appropriate antiserums. At a constant temperature of about 25° C, conjugation between 90G and 60G yielded Fl hybrids with both G serotypes expressed simultaneously. This codominant ex¬ pression in the hybrid phenotype was detectable only after about five fissions. Prior to this stage, the hybrid clones showed only the serotype of the parent from which the cytoplasm was derived. Evidently, the proteins resulting from the new Fl macronucleus replace the preexisting ciliary protein gradually as required during sequential cell divisions. The dual hybrid phenotype persisted
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