The antigenic effect of intravenous injection of diphtheria toxin / by A.T. Glenny and C.G. Pope.

  • Glenny, A. T.
Date:
[1925?]
Catalogue details

Licence: In copyright

Credit: The antigenic effect of intravenous injection of diphtheria toxin / by A.T. Glenny and C.G. Pope. Source: Wellcome Collection.

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    Table I. Showing the antitoxic value of the blood of three rabbits after intravenous and subcutaneous injection of a primary stimulus consisting of a toxin antitoxin mixture. Rabbit G 51. G 52. G 53. Route Intravenous. Intravenous. Subcutaneous. Volume 1-0. 5-0 25 c.c. Antitoxin content in units per c.c. Before injection Nil * Nil Nil \ week after 99 99 9 9 1 „ 99 99 9 9 \\ weeks after 99 99 0-006 2 „ • • • o-ooi 0-006 24 ... 0-002 0-016 0-009 3 „ 0-003 0-02 o-oii 3| ... 0-0045 0-022 0-012 4 „ . . . 0-007 0-025 0-022 41 ... 0-011 0-025 0-022 5 „ 0-012 0-035 0-025 54 „ . . . 0-012 0-035 0-033 6 „ 0-016 0-033 0-033 64 ... 0-022 0-03 0-033 7 „ . . . 0-018 0-022 0-033 74. 0-016 0-022 0-033 8 „ 0-012 0-020 0-033 * Nil means either no antitoxin or less than 0‘0005 unit, i.e., the smallest amount that we ordinarily test for. Normal rabbits injected with concentrated modified toxin have given the following results:— Dose, c.c Route. Maximum antitoxin produced per c.c. of blood. Latent period. 1-0 Subcutaneous O'l unit. 9 days O'l 99 0'04 „ 11 „ 1-0 Intravenous 0-004 „ 5 weeks In a further experiment five normal rabbits were injected sub¬ cutaneously, one with OT c.c. of modified toxin alone and the other * four with a mixture of OT c.c. of modified toxin and antitoxin varying in the different rabbits from 1 unit to 7J units. Six other normal i rabbits were injected intravenously with similar mixtures. The amount of antitoxin produced in the eleven rabbits was scarcely ! detectable. The immunity response to the first stimulus was : therefore judged by the degree of response to a secondary stimulus ] consisting of the subcutaneous injection of OT c.c. of the same: modified toxin. The results given in table II. show that ground immunity is produced