Report on the blood changes following typhoid inoculation / by W. B. Leishman.
- William Boog Leishman
- Date:
- 1905
Licence: In copyright
Credit: Report on the blood changes following typhoid inoculation / by W. B. Leishman. Source: Wellcome Collection.
Provider: This material has been provided by London School of Hygiene & Tropical Medicine Library & Archives Service. The original may be consulted at London School of Hygiene & Tropical Medicine Library & Archives Service.
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![“ C.”—1. Unlysolized Sample. Estimation I. Residue from 15 c.c. IT. n •0031 gm. •0033 „ Av. ^0032 gm. 2. Lijsolizecl Sample (• 5°/o Lysol). Estimation. Residue from 15 c.c. .. .. .. Av. •OOTS gm. The difference which the addition of lysol would make was not anticipated when the estimation of ‘‘B ” was undertaken, so a control experiment was done with two samples of a third vaccine, “ C,” the one unl}^ solized, the other after the addition of '5°/^ lysol. It will be seen from the above figures that the v'^eight of dried bacterial bodies in 15 c.c. of “ A ” and '■ C ” was practically identical and, further, from a comparison of the weight of the lysolized samples of “ B’ and “C” it may fairly be inferred that the weight of bacterial bodies in 15 c.c. of an unlysolized sample of “ B ” would have been about •0033 gm. The weight, then, of dried bacterial bodies in 15 c.c. of each of these three vaccines was as follows:— “A” • • •0033 gm. • • observed. “B” • • •0033 „ • • estimated. “ C ” • . •0032 „ • • observed. Putting the correlation figure obtained from the strong agar emulsions into opeiation, this weight would repiesent approximately 1,700 million bacilli in 1 c.c. of each of these vaccines. I have quoted these experiments at some length from the important bearing they have upon the standardization of the vaccine employed at Aldershot, which was the “ B ” Vaccine of the above series. Owing to the mistake of a subordinate, when sterilizing the rubber caps used for bottling, vaccine “ A ” was contaminated, subsequent to its sterilization and standardization, therefore “ B ” was prepared in precisely the same manner, and, subsequently, “ C,” in order to further control the standardization of “ A ” and “ B ” and to determine][the effect which the addition of lysol had upon the weight of the oacterial sediment. Careful experiments were made with all three vaccines to deter- mine the numbers of germs by dilution (living count) and by Dr. Wright’s blood method, but it was felt that the results obtained were unreliable owing to the presence of numerous clumps of bacilli. The average result of al counts of “ A ” was 750 millions per c.c., and of “ B” 650 millions per c.c., while counts of “ C ” were quite unreliable. To arrive at a piuctical conclusion of the real strength of •* A ” vaccine we put it to the test by inoculating ourselves with different doses, 3 receiving 1 c.c. and the foui’th •! c.c. The local and general reactions in the case of the 1 c.c. dose were severe and prolonged, lasting for 4-5 days, while with the •! c.c. dose](https://iiif.wellcomecollection.org/image/b24916432_0014.jp2/full/800%2C/0/default.jpg)
