Practical pathology : a manual for students and practitioners / by G. Sims Woodhead.
- Woodhead, G. Sims (German Sims), Sir, 1855-1921.
- Date:
- 1892
Licence: Public Domain Mark
Credit: Practical pathology : a manual for students and practitioners / by G. Sims Woodhead. Source: Wellcome Collection.
103/704 page 79
![Mix and allow to stand for one day, then add lo grammes of potash alum; filter as required, using equal parts of stain and of distilled water; stain for five minutes. The great advantage of this fluid is that it is ready for use twenty-four hours after the ingredients are mixed. Add a crystal of thymol, dissolved in alcohol, to prevent the formation of fungi.^ To stain sections, filter about half a drachm of {a) or ten drops of {b) [to {b) add half a drachm of distilled water] into a watch-glass ; allow the sections to remain for about three or four minutes in [a), or half a minute in {b); wash well in water, and mount in Canada balsam (§ 155). JVote.—Never put more than two or three sections at a time into the watch-glass, or they cling together, and are un- equally stained. Should the staining be too intense, place the sections in a watch-glass, pour a few drops of strong acetic acid over them, then wash thoroughly and mount. V. Kahlden recommends that, for the pur- pose of getting rid of over stain, sections should be washed thoroughly in a i per cent, solution of acetic acid, and then for twelve to twenty-four hours in dis- tilled water. {d) For many kinds of work Ehrlich's acid hsematoxylin is greatly to be recommended, especially as a ground work for double and triple staining. Dissolve haematoxylin, 2 parts, in 60 parts absolute alcohol. To this add 6 parts of glycerine and 60 parts of water, both of which are previously saturated with alum, and 3 parts of glacial acetic acid. The great advantage of this solution is that it does not cause over staining nearly so readily as some of the other hsematoxylin preparations. It should be kept exposed to the light for three or four weeks, and then carefully filtered before being used. 104. Eosiiie used as a one-tenth per cent, solution gives a beauti- fully transparent stain, and one which will remain unaltered for a considerable length of time. It may be used in watery solution, especially for muscular tissue of the heart, etc., or as an alcoholic solution for staining the coloured blood corpuscles. It stains nucle A very good logwood staining fluid is made by Martindale, London.](https://iiif.wellcomecollection.org/image/b20388895_0103.jp2/full/800%2C/0/default.jpg)
