Micro-organisms and disease : an introduction into the study of specific micro-organisms / by E. Klein.
- Date:
- 1886
Licence: Public Domain Mark
Credit: Micro-organisms and disease : an introduction into the study of specific micro-organisms / by E. Klein. Source: Wellcome Collection.
Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.
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![*•] A. Gottstein1 places sections of syphilis material for twenty-four hours in fuchsin or aniline oil gentian-violet; wash with distilled water, then place them for a few seconds into a pure or dilute solution of liquor ferri, then wash in alcohol, clarify in clove-oil, mount in Canada-balsam. It may not be unnecessary to point out, that if sections are kept for many hours in the staining fluid, there may be found in them micro- organisms (particularly bacilli) which have been accidentally introduced into them by the solutions of aniline dye. Many of these, particularly when used alkaline, contain organisms, and if the sections are kept in them for many hours, notably in warm weather, bacteria will be found to have not only invaded the tissue but to have multiplied therein. In examining fresh or hardened tissues for micro-organisms it is necessary to make thin sections, which can be easily done with the aid of any of the microtomes in common use, amongst which Williams’s microtome, and especially Dr. Roy’s ether-spray freezing microtome, are no doubt the best and easiest to manipulate. As regards hardened material, it is necessary to remember that the hardening must be carried out properly, small bits—about a half to one cubic inch—of tissue being placed in alcohol, or better, in Muller’s fluid, and kept there; in the first instance, for two to five days; in the second, for from one to three weeks or more. Then small bits are cut out, of which it is desired to make sections. Those hardened in spirit must be soaked well in water to enable the material to freeze, then superficially dried with blotting-paper, and then used for cutting sections with Roy’s microtome. Those hardened in Muller’s fluid are at once superficially dried with blotting-paper and cut. When making sections with Williams’s freezing microtome it is necessary to soak the material first in gum mucilage and then A. Gottstein, Fortschritte d. Medizin, Berlin, 1885, No. 16, p. 545.](https://iiif.wellcomecollection.org/image/b21938325_0033.jp2/full/800%2C/0/default.jpg)
