Report on the influence of glycerine in inhibiting growth of micro-organisms in vaccine lymph / by S. Monckton Copeman and F.R. Blaxall.

  • Sydney Copeman
Date:
1898
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Licence: Public Domain Mark

Credit: Report on the influence of glycerine in inhibiting growth of micro-organisms in vaccine lymph / by S. Monckton Copeman and F.R. Blaxall. Source: Wellcome Collection.

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    i ThE royal society APPENDIX C. LIBRARY Eeports on the Influence of Glycerine, of Lanoline, and of App-c- Vaseline, in Inhibiting the Growth of Micro-organisms On the influence commonly found in Vaccine Lymph ; by Dr, S. Monckton ShibiwthA Oopeman and Dr. F. R. Blaxall. Part I., by Dr. Copeman. Account of the Microbic Constituents of Vaccine Lymph, with History of the various Methods experimentally undertaken, in this and ’Other Countries, for separating from Vaccine Lymph extraneous Micro-organisms, and for preserving indefinitely its Specific Property. In a paper presented to the International Congress of Hygiene held in London in 1891, and subsequently published in the Transactions of that Congress, attention was called to a special method which I had devised for the bacteriological puriiieation and for the preservation of vaccine lymph.# This method consisted in the intimate admixture of a given amount of lymph, or rather vesicle pulp, with twice its quantity of a 50 per cent, solution of chemically pure glycerine in distilled water, and in subsequent storage of the resulting emulsion in sealed capillary tubes for several weeks. For some years antecedent to 1891, I had been engaged in inves¬ tigating the nature and mode of action of the specific virus contained in vaccine lymph, and, in view of the earlier work on this subject by Chauveauf and Burdon SandersonJ having afforded presumption of the particulate nature of the specific virus of vaccinia, my special attention had been directed in the first instance to the bacteriological side of the inquiry. With reference to the literature of the subject, the first account of the discovery of micro-organisms in vaccine lymph and in small pox lymph appears to be that given by Keber§ of Danzig, who evidently regarded the bodies found by him as the carriers, if not the actual generators, of the virulent principle of these diseases. Within the next two years the occurrence of similar bodies in vaccine lymph was described by Burdon Sanderson and by Klebs, In 1872 an important paper was published by Cohnf) of Breslau, in which he treated the morphological aspects of the subject with much complete¬ ness. His observations, which related to both vaccine lymph and variolous lymph, have, as regards the lymph obtained from mature vesicles, at least, received general corroboration from all subsequent workers. He, however, apparently believed the micro-organisms found by him to be of one species only, to which accordingly he gave the name micrococcus vaccinae or variolas, as the case might be, whereas later observers have shown that organisms of more than one species are usually to be found in any given specimen of lymph. Cohn called attention to the fact that in perfectly fresh vaccine lymph the “ corpuscles” for the most part occur singly, but that others * See also Proceedings of Royal Society, vol. 54, p. 189. f Comptes Rendus, lxvi., 1868. J Intimate Pathology of Contagion : 13th Report of the Medical Officer to the Privy Council. § Virchow’s Archiv., xlii., 1868. H Ibid., lv., 1872. e 98312. 100.—11/9* * * §7- W t. 4475. E. & S. A Growth of Micro¬ organisms in Vaccine Lymph; by Drs. Copeman
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