A course of elementary practical physiology / by M. Foster ; assisted by J.N. Langley.

  • Michael Foster
Date:
1884
Catalogue details

Licence: Public Domain Mark

Credit: A course of elementary practical physiology / by M. Foster ; assisted by J.N. Langley. Source: Wellcome Collection.

Provider: This material has been provided by University of Bristol Library. The original may be consulted at University of Bristol Library.

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    b. Filter fresh lemon-juice through flannel and place the tissue in the fi] trate for about 5 minutes before placing it in gold chloride. After treatment with gold chloride, wash the tissue well with water and expose to light in the ordinary way in acidulated water, or place in formic acid in the dark as in (a). (Ranvier.) c. The tissue after treatment with gold chloride is washed and placed in distilled water for 6 to 20 hours until it is of a steel-grey colour ; it is removed to a strong solution of tartaric acid for about 10 minutes and then warmed in the solution up to 40°—50 C. until it becomes dark; this generally takes about ten minutes (Klein). If it is desired to stain the tissue more quickly, it may be left in the gold chloride solution for about three-quarters of an hour, then well washed and at once treated with tartaric acid as above. Silver Nitrate. Por the method of using this cp. Less. xii. A, § 2, C, § 4, and Less, xviii. C, § L It serves mainly to trace out flat tesselated epithelium, such as that of arteries, veins, or lymphatics. By exposure to light, the silver is reduced; in a fresh tissue that has been placed in nitrate of silver, the reduction on exposure takes place more readily in the homogeneous substance between the cells or ' cement substance' than in the cells. As a consequence, where there is a single layer of cells, with a small amount of cement between them, the reduced silver in this substance marks very distinctly the outline of the cells.