Anatomy, physiology, pathology, dictionary / edited by W.A. Evans, Adolph Gehrmann, William Healy.

Date:
1906
Catalogue details

Licence: In copyright

Credit: Anatomy, physiology, pathology, dictionary / edited by W.A. Evans, Adolph Gehrmann, William Healy. Source: Wellcome Collection.

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    then plunged for 15 minutes in van Ermengen's bath (tanno-gallic acid and acetate of soda); wash in water; treat again with silver nitrate solution until the sections are a deep yellow tint; wash in water. Dehydrate, clear and mount in balsam. [The Editor thinks it wise to call attention to the size of spiroehaete. They are nearly as long as the diameter of a red blood corpuscle and they are about one-twentieth as broad. He has seen demonstrations of so-called spirochete which were filaments of fibrin or strings of mucin and traversed much of an immersion oil field.] Observations Upon the Phagocytic Power of the Blood of Supposedly Normal Human Beings. McFarland and L. Engle1 have used the following method: In order to prevent coagulation, the blood is mixed with citrate of sodium, 1 per cent, in .85 per cent, salt solution. A tube 0) inches long with a caliber of 1 millimeter is taken. It is divided into compartments, each about 5 millimeters long. This tube is preceded with a rubber tube and mouthpiece. The citrate solution is drawn to the first mark and the blood next until the whole reaches the 2 mark. The mixture is blown into a receptacle and thoroughly mixed. To this is now added a standardized bacterial suspension in physiologic salt solution. The method of standardizing the solution will be next described. A tube 5 centimeters long is taken. This tube must be about 3 mm. in diameter and rather uniform. The bacterial suspension is drawn up to 5 and then the citratecl blood until the whole stands at 10 cm. They are mixed in the tube or by blowing into a hollow sledge and then sucking back. After being thor- oughly mixed and m the tube the ends are sealed. The tubes are then placed horizontally in an incubator at 37° C. for 30 minutes. The ends of the tube are broken and a small quantity of the mixture is blown on a cover glass and a film is made by using a second cover for smearing. The films are dried in the air and stained with Marino’s stain. The bacteria are counted in 40 nuclear leucocytes and the results are averaged. McFarland and L. Engle’s Nephelometer. This instru- ment is for the purpose of standardizing turbidity in (1) Medicine, April, 1906.