Immune sera; a concise exposition of the main facts and theories of infection and immunity / [Charles Frederick Bolduan].

  • Charles Bolduan
Date:
1911
Catalogue details

Licence: In copyright

Credit: Immune sera; a concise exposition of the main facts and theories of infection and immunity / [Charles Frederick Bolduan]. Source: Wellcome Collection.

    23/276 page 3
    and having been tested for purity by microscopic and culture tests is rendered sterile by the addition of io per cent of a 5 per cent solution of carbolic acid. After 48 hours the dead bacilli have settled on the bottom of the jar and the clear fluid is filtered through ordinary sterile filter paper and stored in full bottles in a cold place until needed. Its strength is then tested by giving a series of guinea pigs carefully measured amounts. Less than 0.01 cc. when injected hypoder- mically should kill a 250 gram guinea pig. Immunizing the Animals. —The horses used should be young, vigorous, of fair size, and absolutely healthy. Vicious habits, such as kicking, etc., make no difference, except, of course, to those who handle the animals. The horses are severally injected with an amount of toxin sufficient to kill five thousand guinea pigs of 250 grams weight (about 20 cc. of strong toxin). After from three to five days, so soon as the fever reaction has subsided, a second subcutaneous injection of a slightly larger dose is given. With the first three injections of toxin 10,000 units of antitoxin are given. If antitoxin is not mixed with the first doses of toxin only one-tenth of the doses advised is to be given. At intervals of from five to eight days increasing injec- tions of pure toxin are made until at the end of two months from ten to twenty times the original amount is given. There is absolutely no way of judging which horses will produce the highest grades of antitoxin. Very roughly those horses which are extremely sensi- tive, and those which react hardly at all are the poorest, but even here there are exceptions. The only way, therefore, is at the end of six weeks or two months to bleed the horses and test their serum. If only high grade serum is wanted all the horses that give less
    digitised image 1
    digitised image 2
    digitised image 3