The cultivation and biological characters of bacillus acnes / by H. J. Südmersen and E. T. Thompson.

  • Südmersen, Henry J.
Date:
[1909]
Catalogue details

Licence: Attribution 4.0 International (CC BY 4.0)

Credit: The cultivation and biological characters of bacillus acnes / by H. J. Südmersen and E. T. Thompson. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    1 per cent, peptone broth and 75 parts, 1 per cent, peptone water; litmus solution is added and neutralisation effected by addition of dilute HC1. Table. Glucose.—Aerobic and anaerobic cultures of both strains give rise to strong acid reaction. In Strain I. the acidity does not appear until after the second or even third week, while Strain II. becomes strongly acid at the end of the first week. An abundant growth occurs. Galactose.—Results throughout the same as with glucose. Saccharose.—A difference is shown in the degree of acidity in the two strains. Strain I. has but little effect, a faint acid reaction is first shown after fourteen days ; this becomes but slightly stronger in three to four weeks. Strain II. produces a markedly acid reaction at the end of the first week. Growth is but slight. Lactose.—Moderate growth. Maltose.—In this medium Strain I. is much less active than Strain II. The latter produces a marked acidity at the end of the first week, the former not until the second or third week of incubation. Raffinose.—This constitutes a differential medium for the two strains; Strain I. causes no change either in aerobic or anaerobic culture. Strain IT., on the contrary, produces a marked acid reaction. Both organisms grow well. Starch.—Strain I. could not be induced to grow in this medium. Strain II. gave rise to a slight degree of acidity in three weeks, which became marked at the end of four weeks. The acidity was stronger and appeared earlier aerobically. Inulin.—Strain I. gave slight acid at the end of fourteen days in both aerobic and anaerobic culture; in the latter bleaching occurred in twenty-one days. Strain II. gave stronger acidity without bleaching. Arabinose.—Both strains gave marked acidity in aerobic and anaerobic culture. This was shown after seven days, being somewhat stronger in Strain II. Growth is moderate. Amygdalin.—Appears to be unaffected; growth is slight. Arbutin.—Was unaffected; growth slight. Salicin.—Neither strain appeared to grow in this medium. Glycerin.—A strong acid reaction appeared in Strain II. within seven days, in Strain I. not until the end of the second week. The reaction occurs earlier in aerobic culture. Growth is moderate. Erythrol and Dulcite.—Slight growth.
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