On the fractionation of agglutinins and antitoxin / by Robert Banks Gibson and Katharine R. Collins.

  • Gibson, Robert Banks.
Date:
1907
Catalogue details

Licence: In copyright

Credit: On the fractionation of agglutinins and antitoxin / by Robert Banks Gibson and Katharine R. Collins. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

    4/22 page 234
    The literature on the fractional precipitation of the antibodies is not extensive. Ide and Lemaire1 (in 1899) found the precipi- tation limits of diphtheria antitoxin in horse serum to be from 2.8-4.4 saturation. Fuld and Spiro2 (1900) associated the anti- rennin of horse serum with the pseudoglobulin, while a milk- coagulating action was possessed by the eu-fraction. The fail- ures of Brodie and of Atkinson (of this laboratory) to separate diphtheria antitoxin from the accompanying serum globulins are referred to in the following paper. Porges and Spiro3 without giving any of their experimental protocols divide, according to the distribution of the antibodies, the senim globulin into three distinct fractions; the ammonium sulphate precipitation bound- aries of these overlap unless the serum is greatly diluted. Land- steiner4 5 * found that the antitryptic action of blood serum is pos- sessed by the albumin precipitated by complete saturation with ammonium sulphate after removal of the globulin. Cathcartr’ also observed the antitrypsin to be associated with the albumin but not with the globulin fraction. Glaessner0 states that the euglobulin fraction inhibits the action of trypsin, but the typical protocol which he publishes and his statement of the Hofmeister classification show a misconception and confusion of the identity of his fractions.7 Glaessner apparently found that the globulin remaining in solution on dialysis was antitryptic. Very recently Simon, Lamar and Bispham8 found that the opsonic substance in blood serum was precipitated with the serum 1 Ide and Lemaire: Arch, internat. d. pliarmacodyn., vi, p. 477, 1899. 1 Fuld and Spiro: Zeilschr. f. physiol. Chem., xxxi, p. 133, 1900. 3 Porges and Spiro: Beitr. z. chem. Physiol, u. Path., iii, p. 277, 1903 4 Landsteiner: Centralbl. f. Bakt., xxvii, Abt. I, p. 357, 1900. 5 Cathcart: Journ. of Physiol., xxxi, p. 497, 1904. 0 Glaessner: Beitr. z. chem. Physiol, u. Path., iv, p. 79, 1904. 7 The error on his part has not heretofore been noted. Glaessner states (p. 82): “ Das Globulin des Blutserums l&sst sich nach den in Hofmeisters Laboratorium in mindestens 3 Fractionen zerlegen: in das bei 25 Proz. Sattigung mit Ammonsulfat ausfallbare Fibrino-globulin, in das Euglo- bulin, das bei einer Sattigung von 33 Proz. ausfallt und bei der Dialyse in Lbsung bleibt, und endlich in das bei 38 Proz. Sattigung ausfallbare bei der Dialyse unlosliche Pseudoglobulin.” The confused nomenclature of the degrees of salt saturation is discussed in the following paper (p. 254). '“Simon, Lamar and Bispham: Journ. of Exp. Med., viii, p. 651, 1906.
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