Serum diagnosis of syphilis and the butyric acid test for syphilis / by Hideyo Noguchi.

Date:
[1911]
Catalogue details

Licence: In copyright

Credit: Serum diagnosis of syphilis and the butyric acid test for syphilis / by Hideyo Noguchi. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

    85/268 page 65
    inhibit hgemolysis, while the front tube, in which haemolysis is inhibited, shows the ability of syphilitic antibody to fix complement in the presence of the antigen employed. These essentials having been fulfilled, the tubes containing serum for diagnosis can be scrutinized. •Seifordiegnoii. Positive controlf' ^Negdt/]/e , ' contro/ y <^ set «lL_]lli <110!ll> <liir~«llti fflijrj -<1I|QJ» <ilIOll||> eg}.—Finn Antigen^ -O ^^j^ Amboceptor AnfigenX<> Antic, \^j-~AmboceptOi wboceptor Fig. 10.—This picture shows the appearance of the tubes after completion of haemolysis, namely, after the second incubation. In all front tubes there are two square pieces of paper, one representing antigen and the other amboceptor. In each rear tube there is but one piece, and it represents amboceptor. In negative control set haemolysis occurred in both tubes. In positive control set haemolysis took place in the rear tube only and not in the front. In the set for diagnosis the conditions are seen to be identi- cal with the positive control set, hence this serum is found to be syphilitic. In these tests haemolysis must be complete in the rear row, since antigen is not present and the amount of serum used should not be inhibitory. Should haemol- ysis be inhibited markedly, showing usually, an anti- complementary action on the part of the patient's serum, this may be overcome by inactivation of the serum for 20 minutes at 56° C. (before com-
    digitised image 1
    digitised image 2
    digitised image 3